{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T00:09:17Z","timestamp":1767830957235,"version":"3.49.0"},"reference-count":11,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,1,4]],"date-time":"2022-01-04T00:00:00Z","timestamp":1641254400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate bone mineral density (BMD), bone metabolism\u2010related factors, and microRNA\u2010218 in Chinese ankylosing spondylitis (AS) patients and to identify their correlation with disease activities and the treatment with TNF\u2010\u03b1 inhibitors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 89 AS patients were enrolled in the study. Patients\u2019 information and laboratory examination results were collected. BMD of the anteroposterior lumbar spine (L2\u2010L4), left femoral neck, and whole body were measured and T\u2010scores were calculated. MicroRNA\u2010218 was extracted from PBMCs of AS patients and detected by RT\u2010PCR. Bone metabolism\u2010related factors were detected using protein chips and flow cytometer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Out of 86 patients undergoing whole\u2010body BMD measurement, 14\u00a0had osteopenia and 72\u00a0had normal BMD without osteoporosis or high BMD. Compared with short\u2010 (disease duration \u22643\u00a0years) and long\u2010term groups (disease duration \u226510\u00a0years), medium\u2010term group (disease duration ranges from 3 to 10\u00a0years) showed lowest BMD. Patients with onset age \u226420\u00a0years old had significantly lower BMD than the other groups (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). The BMD of femoral neck had negative correlation with CRP (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05) and no correlation with BASDAI or ESR. Both whole\u2010body BMD and femoral neck BMD were negatively correlated with BASMI (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). Dickkopf\u20101 (DKK\u20101), platelet\u2010derived growth factor\u2010BB (PDGF\u2010BB), and receptor activator of NF\u2010\u03baB ligand (RANKL)\/osteoprotegerin (OPG) were significantly increased, while Osteopontin (OPN) was significantly decreased in AS patients. Expression of microRNA\u2010218 in PBMC of AS patients was low and was positively correlated with BASMI (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05), but it was not correlated with the duration of disease, age of onset, BASDAI, ESR, or BMD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Loss of bone mass mainly occurred at the inflammatory sites in AS patients, depending on the severity of inflammation. The alleviation of inflammation can improve loss of bone mass and bone metabolism disorders. Anti\u2010inflammatory treatment is critical for the treatment of secondary osteoporosis caused by AS.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24223","type":"journal-article","created":{"date-parts":[[2022,1,5]],"date-time":"2022-01-05T07:31:55Z","timestamp":1641367915000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Bone mineral density, bone metabolism\u2010related factors, and microRNA\u2010218 are correlated with disease activities in Chinese ankylosing spondylitis patients"],"prefix":"10.1002","volume":"36","author":[{"given":"Jiayan","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Rheumatology Jiading District Central Hospital Affiliated Shanghai University of Medicine &amp; Health Sciences Shanghai China"},{"name":"Institute of Rheumatology, Immunology and Allergy Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Rheumatology, Immunology and Allergy Fudan University Shanghai China"},{"name":"Department of Rheumatology Huashan Hospital Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xue","family":"Yang","sequence":"additional","affiliation":[{"name":"Institute of Rheumatology, Immunology and Allergy Fudan University Shanghai China"},{"name":"Department of Rheumatology Huashan Hospital Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Congjin","family":"Liu","sequence":"additional","affiliation":[{"name":"Institute of Rheumatology, Immunology and Allergy Fudan University Shanghai China"},{"name":"Department of Nuclear Medicine Huashan Hospital Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ning","family":"Kong","sequence":"additional","affiliation":[{"name":"Institute of Rheumatology, Immunology and Allergy Fudan University Shanghai China"},{"name":"Department of Rheumatology Huashan Hospital Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yiyun","family":"Yu","sequence":"additional","affiliation":[{"name":"Institute of Rheumatology, Immunology and Allergy Fudan University Shanghai China"},{"name":"Department of Rheumatology Huashan Hospital Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dandan","family":"Xuan","sequence":"additional","affiliation":[{"name":"Institute of Rheumatology, Immunology and Allergy Fudan University Shanghai China"},{"name":"Department of Rheumatology Huashan Hospital Fudan University Shanghai 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China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,1,4]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2015-208174"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0b013e3283620777"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0099685"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1159\/000235897"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar3833"},{"issue":"3","key":"e_1_2_11_7_1","first-page":"502","article-title":"The effect of biologic agents on bone homeostasis in chronic inflammatory rheumatic diseases","volume":"37","author":"Di Munno O","year":"2019","journal-title":"Clin Exp 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