{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:56:17Z","timestamp":1753869377949,"version":"3.41.2"},"reference-count":19,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2022,1,8]],"date-time":"2022-01-08T00:00:00Z","timestamp":1641600000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Introduction<\/jats:title><jats:p>RT\u2010PCR is widely used as a diagnostic test for the detection of SARS\u2010CoV\u20102. In this study, we aim to describe the clinical utility of serial PCR testing in the final detection of COVID\u201019.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>We collected multiple nasopharyngeal swab samples from patients who had negative RT\u2010PCR test on the first day after hospitalization. RT\u2010PCR tests were performed on the second day for all patients with initial negative result. For the patients with secondary negative results on day 2, tertiary RT\u2010PCR tests were performed on day 3 after hospitalization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Result<\/jats:title><jats:p>Among 68 patients with initial negative test results, at the end of follow\u2010up, the mortality number was 20 (29.4%). About 33.8% of patients had subsequent positive PCR test results for the second time and 17.4% of the patients who performed third PCR test had positive result.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Based on this study, serial RT\u2010PCR testing is unlikely to yield additional information.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24226","type":"journal-article","created":{"date-parts":[[2022,1,8]],"date-time":"2022-01-08T10:22:00Z","timestamp":1641637320000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["One\u2010step and sequential SARSCOV\u20102 polymerase chain reaction tests would not work every time"],"prefix":"10.1002","volume":"36","author":[{"given":"Firouze","family":"Hatami","sequence":"first","affiliation":[{"name":"Department of Infectious Diseases and Tropical Medicine Loghman Hakim Hospital Shahid Beheshti University of Medical Sciences Tehran Iran"},{"name":"Infectious Diseases and Tropical Medicine Research Center Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohammad Mahdi","family":"Rabiei","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Tropical Medicine Loghman Hakim Hospital Shahid Beheshti University of Medical Sciences Tehran Iran"},{"name":"Infectious Diseases and Tropical Medicine Research Center Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Farid","family":"Javandoust Gharehbagh","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Tropical Medicine Loghman Hakim Hospital Shahid Beheshti University of Medical Sciences Tehran Iran"},{"name":"Infectious Diseases and Tropical Medicine Research Center Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohamad Amin","family":"Pourhoseingholi","sequence":"additional","affiliation":[{"name":"Gastroenterology and Liver Diseases Research Center Research Institute for Gastroenterology and Liver Diseases Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shahram","family":"Sabeti","sequence":"additional","affiliation":[{"name":"Pathology Ward Loghman Hakim Hospital Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mahnaz","family":"Kheyrian","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Tropical Medicine Loghman Hakim Hospital Shahid Beheshti University of Medical Sciences Tehran Iran"},{"name":"Infectious Diseases and Tropical Medicine Research Center Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4440-335X","authenticated-orcid":false,"given":"Ilad","family":"Alavi Darazam","sequence":"additional","affiliation":[{"name":"Department of Infectious Diseases and Tropical Medicine Loghman Hakim Hospital Shahid Beheshti University of Medical Sciences Tehran Iran"},{"name":"Infectious Diseases and Tropical Medicine Research Center Shahid Beheshti University of Medical Sciences Tehran Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,1,8]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abc6156"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpha.2020.02.010"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms17111880"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/14737159.2020.1757437"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01695-20"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25721"},{"key":"e_1_2_9_8_1","unstructured":"National Institutes of Health (NIH)Clinical presentation of people with SARS\u2010CoV\u20102 infection. 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