{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,12]],"date-time":"2026-01-12T22:55:05Z","timestamp":1768258505634,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,2,5]],"date-time":"2022-02-05T00:00:00Z","timestamp":1644019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Calprotectin is a calcium\u2010binding protein that can be measured in serum, plasma, and feces. Increased serum and plasma calprotectin concentrations have been found in chronic inflammatory rheumatic disorders. An analytical and clinical evaluation of the DiaSorin Liaison\u00ae fecal Calprotectin assay using LIAISON\u00ae XL was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The protocol included an analytical and clinical evaluation in which imprecision, the linearity of dilution, differences between serum and plasma samples and method comparison with CalproLab\u2122 ELISA kit were assessed. Serum calprotectin concentrations in active (<jats:italic>n<\/jats:italic>\u00a0=\u00a026) and remission (<jats:italic>n<\/jats:italic>\u00a0=\u00a023) rheumatoid arthritis (RA) patients were compared.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The intra\u2010day and inter\u2010day analytical imprecision CVs ranged from 2.9% to 4.0% and 2.7% to 10.4%, respectively. Correlation between measured and expected values was high (<jats:italic>R<\/jats:italic>\u00a0&gt;\u00a00.99), indicating good linearity. The Wilcoxon signed\u2010rank test showed that serum and plasma matched samples presented statistically significant differences (<jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.001) being the highest concentrations of calprotectin observed in serum samples. Deming regression equation was as follows: Diasorin calprotectin (\u03bcg\/ml)\u00a0=\u00a0\u22120.32 (95% CI: \u22120.65 \u2010 \u22120.05) +1.58 (95% CI: 1.42\u20131.79).* Calprolab calprotectin (\u03bcg\/ml). Significantly higher serum calprotectin levels were found in RA patients with active disease when compared to patients with low disease activity or in clinical remission (mean\u00a0\u00b1\u00a0SD) [(3.35\u00a0\u03bcg\/ml\u00a0\u00b1\u00a01.55) vs. (1.63\u00a0\u03bcg\/ml\u00a0\u00b1\u00a00.52), <jats:italic>p\u00a0<\/jats:italic>&lt;\u00a00.001] and these levels correlated well with all disease activity indices.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The DiaSorin Liaison\u00ae fecal Calprotectin assay adapted for serum samples showed adequate technical performances and the clinical performances were similar to other assays.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24258","type":"journal-article","created":{"date-parts":[[2022,2,5]],"date-time":"2022-02-05T09:42:22Z","timestamp":1644054142000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Analytical and clinical evaluation of DiaSorin Liaison\u00ae Calprotectin fecal assay adapted for serum samples"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8314-3087","authenticated-orcid":false,"given":"Laura","family":"Macias\u2010Mu\u00f1oz","sequence":"first","affiliation":[{"name":"Biochemistry and Molecular Genetics Department Hospital Clinic of Barcelona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatriz","family":"Frade\u2010Sosa","sequence":"additional","affiliation":[{"name":"Arthritis Unit Rheumatology Department Hospital Clinic of Barcelona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose","family":"Iniciarte\u2010Mundo","sequence":"additional","affiliation":[{"name":"Arthritis Unit Rheumatology Department Hospital Clinic of Barcelona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susana","family":"Hidalgo","sequence":"additional","affiliation":[{"name":"Biochemistry and Molecular Genetics Department Hospital Clinic of Barcelona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa Maria","family":"Morla","sequence":"additional","affiliation":[{"name":"Arthritis Unit Rheumatology Department Hospital Clinic of Barcelona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yadira","family":"Gallegos","sequence":"additional","affiliation":[{"name":"Biochemistry and Molecular Genetics Department Hospital Clinic of Barcelona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raimon","family":"Sanmarti","sequence":"additional","affiliation":[{"name":"Arthritis Unit Rheumatology Department Hospital Clinic of Barcelona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6383-7308","authenticated-orcid":false,"given":"Josep Maria","family":"Auge","sequence":"additional","affiliation":[{"name":"Biochemistry and Molecular Genetics Department Hospital Clinic of Barcelona Barcelona Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,2,5]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cccn.2004.02.023"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2004-02-0446"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.26.753"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200003)43:3<628::AID-ANR20>3.0.CO;2-X"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2006.064741"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/JIM.0000000000000095"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2013.11.003"},{"key":"e_1_2_6_9_1","first-page":"2239","article-title":"Calprotectin more accurately discriminates the disease status of rheumatoid arthritis patients receiving tocilizumab than acute phase 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clinical performance of the fully\u2010automated LIAISONXL calprotectin immunoassay from DiaSorin in IBD patients","volume":"21","author":"Vicente\u2010Steijn R","year":"2020","journal-title":"Pract Lab Med"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-61094-z"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2017-211695"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1177\/1535370216681551"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2261-14-196"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365521.2019.1665097"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12950-015-0090-3"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1080\/00365513.2018.1550810"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1373\/jalm.2017.024778"},{"key":"e_1_2_6_28_1","first-page":"859","article-title":"Calprotectin in 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