{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T09:33:56Z","timestamp":1788860036064,"version":"build-2803163510"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,2,11]],"date-time":"2022-02-11T00:00:00Z","timestamp":1644537600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Mycoplasma pneumoniae<\/jats:italic> (MP) is a common pathogen of community\u2010acquired pneumonia in children. In the present study, serum amyloid A (SAA), C\u2010reactive protein (CRP), and procalcitonin (PCT) levels in children with MP infection were analyzed and the differential diagnoses of MP evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study included 152 children with MP infection hospitalized in Tai\u2019an Central Hospital in Shandong Province and 50 healthy children as controls. SAA, CRP, and PCT, as well as serum immunoglobulins and T lymphocyte subsets were analyzed during the acute and convalescent phases. Among the MP\u2010infected children, 30 cases were selected to monitor the SAA, immunoglobulins, and T lymphocyte subset levels for a week.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The SAA, CRP, PCT, IgA, and IgM levels were significantly higher in the MP\u2010infected group than in the control group (<jats:italic>F<\/jats:italic><jats:sub>(SAA)<\/jats:sub> = 83.91, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05; <jats:italic>F<\/jats:italic><jats:sub>(CRP)<\/jats:sub> = 40.79, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05; <jats:italic>F<\/jats:italic><jats:sub>(PCT)<\/jats:sub> = 60.58, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05; <jats:italic>F<\/jats:italic><jats:sub>(IgA)<\/jats:sub> = 43.45, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05; <jats:italic>F<\/jats:italic><jats:sub>(IgM)<\/jats:sub> = 233.88, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). In addition, the levels of these factors were significantly higher in the acute phase than in the convalescent phase (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). However, significant difference was not observed in the IgG level between these two groups (<jats:italic>p<\/jats:italic>\u00a0&gt;\u00a00.05). The CD3<jats:sup>+<\/jats:sup> and CD4<jats:sup>+<\/jats:sup> levels in the MP\u2010infected group were lower than in the control group ( <jats:italic>F<\/jats:italic><jats:sub>(CD3+)<\/jats:sub>= 60.58, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05; <jats:italic>F<\/jats:italic><jats:sub>(CD4+)<\/jats:sub> = 89.05, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05), and the CD8<jats:sup>+<\/jats:sup> level was higher than in the control group ( <jats:italic>F<\/jats:italic><jats:sub>(CD8+)<\/jats:sub>= 96.96, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). The CD3<jats:sup>+<\/jats:sup>, CD4<jats:sup>+<\/jats:sup>, and CD8<jats:sup>+<\/jats:sup> levels were significantly different between the acute phase and the convalescent phase (CD3<jats:sup>+<\/jats:sup>: acute phase <jats:italic>vs<\/jats:italic>. convalescent phase, <jats:italic>q<\/jats:italic>\u00a0=\u00a02.79, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05; CD4<jats:sup>+<\/jats:sup>: acute phase <jats:italic>vs<\/jats:italic>. convalescent phase, <jats:italic>q<\/jats:italic>\u00a0=\u00a02.83, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05; CD8<jats:sup>+<\/jats:sup>: acute phase <jats:italic>vs<\/jats:italic>. convalescent phase, <jats:italic>q<\/jats:italic>\u00a0=\u00a03.15, <jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). The changes in serum SAA levels in the MP\u2010infected group positively correlated with the changes in IgA, IgM, and CD8<jats:sup>+<\/jats:sup> levels and negatively correlated with CD3<jats:sup>+<\/jats:sup>, CD4<jats:sup>+<\/jats:sup>, and CD4<jats:sup>+<\/jats:sup>\/CD8<jats:sup>+<\/jats:sup>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>SAA, CRP, and PCT were specific markers for diagnosing early MP infection in children. These findings are important in the differential diagnosis of MP infection and clinical guidance for MP treatment.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24265","type":"journal-article","created":{"date-parts":[[2022,2,11]],"date-time":"2022-02-11T13:53:05Z","timestamp":1644587585000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Serum amyloid a, C\u2010reactive protein, and procalcitonin levels in children with <i>Mycoplasma pneumoniae<\/i> infection"],"prefix":"10.1002","volume":"36","author":[{"given":"Yuanyuan","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Taian City Central Hospital  Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenyang","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Taian City Central Hospital  Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhijun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Taian City Central Hospital  Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xianfen","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Taian City Central Hospital  Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanyan","family":"Sang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Taian City Central Hospital  Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Taian City Central Hospital  Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guoxiang","family":"Xu","sequence":"additional","affiliation":[{"name":"Shanghai Upper Bio Tech Pharma Co., Ltd  Shanghai China"},{"name":"Shanghai Engineering Research Center of iPOCT Medicine  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Feng","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Taian City Central Hospital  Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuping","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Clinical Laboratory Taian City Central Hospital  Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,2,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.24963"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00296-016-3626-9"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0000000000000494"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.14715\/cmb\/2020.66.6.8"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-030-41769-7_17"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41372-019-0363-4"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2019-0194"},{"key":"e_1_2_8_9_1","volume-title":"Zhu Futang Practice of Pediatrics","author":"Zaifang J","year":"2017"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001432-200104000-00012"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0156465"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00508-006-0563-8"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/omi.12225"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2020.10.006"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chest.2016.08.201"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00114-16"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.23095"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ppul.20961"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jiac.2015.12.006"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2014.07.020"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cca.2020.01.004"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2020.105921"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.22887"},{"issue":"5","key":"e_1_2_8_24_1","first-page":"490","article-title":"Increased Total Serum Immunoglobulin E in Children Developing Mycoplasma pneumoniae\u2010related Extra\u2010pulmonary Diseases","volume":"17","author":"Poddighe D","year":"2018","journal-title":"Iran J Allergy Asthma Immunol"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.24265","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.24265","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.24265","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,27]],"date-time":"2023-08-27T05:31:37Z","timestamp":1693114297000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.24265"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,11]]},"references-count":23,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2022,3]]}},"alternative-id":["10.1002\/jcla.24265"],"URL":"https:\/\/doi.org\/10.1002\/jcla.24265","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,2,11]]},"assertion":[{"value":"2021-06-04","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-01-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-02-11","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e24265"}}