{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,3]],"date-time":"2026-11-03T17:22:36Z","timestamp":1793726556442,"version":"4.6.1"},"reference-count":26,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2022,2,10]],"date-time":"2022-02-10T00:00:00Z","timestamp":1644451200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Purpose<\/jats:title>\n                    <jats:p>The aim of this study was to explore the value of the combination between lung ultrasound score (LUS) and the expression of plasma miR\u201021\u20103p in predicting the prognosis of patients with acute lung injury (ALI).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Patients and methods<\/jats:title>\n                    <jats:p>\n                      A total of 136 ALI patients were divided into survival (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a086) and death groups (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a050), or into low\/middle\u2010risk (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a077) and high\u2010risk groups (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a059) according to APACHE II scores. Bioinformatics was used to explore the mechanism of action of miR\u201021\u20103p in humans. Real\u2010time fluorescent quantitative PCR was used to detect the expression of miR\u201021\u20103p in plasma, and LUS was recorded. Receiver operator characteristic (ROC) curve and Pearson correlation were also used.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The LUS and expression level of plasma miR\u201021\u20103p in the death and high\u2010risk groups were significantly higher than those in the survival and low\/middle\u2010risk groups (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.01 and\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05). miR\u201021\u20103p expression leads to pulmonary fibrosis and promotes the deterioration of ALI patients by regulating fibroblast growth factor and other target genes. ROC curve analysis showed that the best cutoff values for LUS and plasma miR\u201021\u20103p expression were 18.60 points and 1.75, respectively. LUS score and miR\u201021\u20103p combined predicted the death of ALI patients with the largest area under the curve (0.907, 95% CI: 0.850\u20130.964), with sensitivity and specificity of 91.6% and 85.2%, respectively. The expression level of plasma miR\u201021\u20103p was positively correlated with LUS in the death group (\n                      <jats:italic>r<\/jats:italic>\n                      \u00a0=\u00a00.827,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.01).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>LUS and expression level of miR\u201021\u20103p in plasma are correlated with the severity and prognosis of ALI patients, and their combination has a high value for the prognostic assessment of ALI patients.<\/jats:p>\n                  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