{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:56:20Z","timestamp":1753869380738,"version":"3.41.2"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,2,17]],"date-time":"2022-02-17T00:00:00Z","timestamp":1645056000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004320","name":"Shiraz University of Medical Sciences","doi-asserted-by":"publisher","award":["97\u201001\u201032\u201017090"],"award-info":[{"award-number":["97\u201001\u201032\u201017090"]}],"id":[{"id":"10.13039\/501100004320","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The present study aimed to explore the changes in the expressions of six tumor\u2010related genes in myeloproliferative neoplasms (MPNs). The study population included 130 patients with MPNs (52 with chronic myeloid leukemia (CML), 49 with essential thrombocythemia (ET), 20 with polycythemia vera (PV), and 9 with primary myelofibrosis (PMF)) and 51\u00a0healthy individuals.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The expression profiling of six genes (<jats:italic>ADAMTS18<\/jats:italic>, <jats:italic>CMTM5<\/jats:italic>, <jats:italic>CDKN2B<\/jats:italic>, <jats:italic>DCC<\/jats:italic>, <jats:italic>FHIT<\/jats:italic>, and <jats:italic>WNT5B<\/jats:italic>) in the peripheral blood granulocyte cells was explored by real\u2010time quantitative reverse transcription polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The patients with MPNs showed significant downregulation of <jats:italic>CMTM5<\/jats:italic> (EFC\u00a0=\u00a00.66) and <jats:italic>DCC<\/jats:italic> (EFC\u00a0=\u00a00.65) genes in contrast to a non\u2010significant upregulation of <jats:italic>ADAMTS18<\/jats:italic>, <jats:italic>CDKN2B<\/jats:italic>, <jats:italic>FHIT<\/jats:italic>, and <jats:italic>WNT5B<\/jats:italic> genes. Downregulation of <jats:italic>DCC<\/jats:italic> was consistent in all subtypes of MPN (EFC range: 0.591\u20130.860). However, <jats:italic>CMTM5<\/jats:italic>\u00a0had a 1.22\u2010fold upregulation in PMF in contrast to downregulation in other MPN subtypes (EFC range: 0.599\u20130.775). The results revealed a significant downregulation in <jats:italic>CMTM5<\/jats:italic> and <jats:italic>DCC<\/jats:italic> at below 60\u2010years of age. Furthermore, female patients showed a clear\u2010cut downregulation in both <jats:italic>CMTM5<\/jats:italic> and <jats:italic>DCC<\/jats:italic> (EFC <jats:italic>DCC<\/jats:italic>: 0.436 and <jats:italic>CMTM5<\/jats:italic>: 0.570), while male patients presented a less prominent downregulation with a borderline <jats:italic>p<\/jats:italic>\u2010value only in <jats:italic>DCC<\/jats:italic> (EFC: 0.69; <jats:italic>p<\/jats:italic>\u00a0=\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Chronic myeloid leukemia cases showed a significant upregulation of <jats:italic>WNT5B<\/jats:italic>, as a known oncogenesis gene. Two tumor suppressor genes, namely <jats:italic>DCC<\/jats:italic> and <jats:italic>CMTM5<\/jats:italic>, were downregulated in the patients with MPNs, especially in females and patients below 60\u00a0years of age.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24289","type":"journal-article","created":{"date-parts":[[2022,2,17]],"date-time":"2022-02-17T17:54:52Z","timestamp":1645120492000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Oncogenic and tumor suppressor genes expression in myeloproliferative neoplasms: The hidden side of a complex pathology"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0304-1452","authenticated-orcid":false,"given":"Elham","family":"Abedi","sequence":"first","affiliation":[{"name":"Hematology Research Center Shiraz University of Medical Sciences  Shiraz 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