{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T15:10:19Z","timestamp":1790694619504,"version":"4.1.0"},"reference-count":39,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T00:00:00Z","timestamp":1644969600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004826","name":"Natural Science Foundation of Beijing Municipality","doi-asserted-by":"publisher","award":["L202004"],"award-info":[{"award-number":["L202004"]}],"id":[{"id":"10.13039\/501100004826","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100017501","name":"Science-Health Joint Medical Scientific Research Project of Chongqing","doi-asserted-by":"publisher","award":["2016ZDXM041"],"award-info":[{"award-number":["2016ZDXM041"]}],"id":[{"id":"10.13039\/100017501","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Some streptococci strains identified as <jats:italic>Streptococcus pneumoniae<\/jats:italic> (<jats:italic>S.\u00a0pneumoniae<\/jats:italic>) by routine clinical methods exhibiting negative Quellung reaction results may belong to other species of viridans group streptococci or non\u2010typeable <jats:italic>S.\u00a0pneumoniae<\/jats:italic>. The purpose of this study was to investigate the identification and molecular characteristics of <jats:italic>S.\u00a0pneumoniae<\/jats:italic> with negative Quellung reaction results.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>One hundred and five isolates identified as <jats:italic>S.\u00a0pneumoniae<\/jats:italic> using routine microbiological methods with negative Quellung reaction results were included. Multilocus sequence analysis (MLSA) was used as a gold standard in species identification, and the capacity of matrix\u2010assisted laser desorption ionization\u2010time of flight mass spectrometry (MALDI\u2010TOF MS) in identification was evaluated. Capsular genes and sequence types of <jats:italic>S.\u00a0pneumoniae<\/jats:italic> isolates were determined by sequential multiplex PCR and multilocus sequence typing. Antimicrobial susceptibility patterns were determined via broth microdilution with a commercialized 96\u2010well plate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among the isolates, 81 were identified as <jats:italic>S.\u00a0pneumoniae<\/jats:italic> and 24 were <jats:italic>S.\u00a0pseudopneumoniae<\/jats:italic> by MLSA. MALDI\u2010TOF MS misidentified six <jats:italic>S.\u00a0pneumoniae<\/jats:italic> isolates as <jats:italic>S.\u00a0pseudopneumoniae<\/jats:italic> and nine <jats:italic>S.\u00a0pseudopneumoniae<\/jats:italic> isolates as <jats:italic>S.\u00a0pneumoniae<\/jats:italic> or <jats:italic>S.\u00a0mitis<\/jats:italic>\/<jats:italic>S.\u00a0oralis<\/jats:italic>. Thirty\u2010one sequence types (STs) were detected for these 81 <jats:italic>S.\u00a0pneumoniae<\/jats:italic> isolates, and the dominant ST was ST\u2010bj12 (16, 19.8%). The non\u2010susceptibility rates of <jats:italic>S.\u00a0pseudopneumoniae<\/jats:italic> were comparable to those of NESp strains.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Some <jats:italic>S.\u00a0pneumoniae<\/jats:italic> isolates identified by routine methods were <jats:italic>S.\u00a0pseudopneumoniae<\/jats:italic>. Most NESp strains have a different genetic background compared with capsulated <jats:italic>S.\u00a0pneumoniae<\/jats:italic> strains. The resistance patterns of <jats:italic>S.\u00a0pseudopneumoniae<\/jats:italic> against common antibiotics were comparable to those of NESp.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24293","type":"journal-article","created":{"date-parts":[[2022,2,16]],"date-time":"2022-02-16T09:06:22Z","timestamp":1645002382000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Identification and molecular epidemiology of routinely determined <i>Streptococcus pneumoniae<\/i> with negative Quellung reaction results"],"prefix":"10.1002","volume":"36","author":[{"given":"Ju","family":"Jia","sequence":"first","affiliation":[{"name":"Beijing Key Laboratory of Pediatric Respiratory Infection Diseases Key Laboratory of Major Diseases in Children Ministry of Education National Clinical Research Center for Respiratory Diseases National Key Discipline of Pediatrics (Capital Medical University) Beijing Pediatric Research Institute Beijing 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