{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T16:58:22Z","timestamp":1790960302814,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,3,25]],"date-time":"2022-03-25T00:00:00Z","timestamp":1648166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100014717","name":"National Outstanding Youth Science Fund Project of National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["180530068"],"award-info":[{"award-number":["180530068"]}],"id":[{"id":"10.13039\/100014717","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Early diagnosis of pancreatic ductal adenocarcinoma (PDAC) is difficult due to the lack of effective screening tests. CA199, the standard biomarker for PDAC management, is not sufficiently reliable for early diagnosis. This prospective study aimed to evaluate whether circulating tumor cells (CTCs) could complement or perform better than CA199 in determining PDAC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 168 blood samples were collected from 80 patients with PDAC, 32 patients with acute pancreatitis, 22 patients with benign pancreatic masses, and 34\u00a0healthy donors. CTCs were detected by a novel system combining negative enrichment with immunostaining and fluorescence in situ hybridization (NE\u2010imFISH). Next, ROC curves and AUC analyses were conducted to assess diagnostic abilities of CA199, CTCs, and the combination of the two biomarkers in PDAC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CTCs were stained as CD45\u2013\/DAPI+\/CEP8 \u22653. With 2 CTCs\/3.2\u00a0ml as the cut\u2010off value, the sensitivity\/specificity of the CTC number was 0.76\/0.94, which was comparable to that of CA199 (0.78\/0.83; Delong test <jats:italic>p<\/jats:italic>\u00a0=\u00a00.3360). Improved performance was achieved through a logistic regression model integrating CA199 and CTC number (AUC<jats:sub>CTC+CA199<\/jats:sub>\u00a0=\u00a00.95, AUC<jats:sub>CA199<\/jats:sub>\u00a0=\u00a00.80, AUC<jats:sub>CTC number<\/jats:sub>\u00a0=\u00a00.85; Delong test <jats:italic>p<\/jats:italic>\n<jats:sub>vs<\/jats:sub>. <jats:sub>CA199<\/jats:sub>\u00a0&lt;\u00a00.0001 and <jats:italic>p<\/jats:italic>\n<jats:sub>vs<\/jats:sub>. <jats:sub>CTC number<\/jats:sub>\u00a0=\u00a00.0002). CTC subtype was inferior to CTC number as a diagnostic marker (AUC<jats:sub>CTC subtype<\/jats:sub>\u00a0=\u00a00.73; Delong test <jats:italic>p<\/jats:italic>\n<jats:sub>vs<\/jats:sub>. <jats:sub>CTC number<\/jats:sub>\u00a0&lt;\u00a00.0001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The dual\u2010marker panel consisting of CA199 and CTC number can significantly improve upon the diagnostic performance of CA199 alone, highlighting the promising clinical utilization as an effective strategy for PDAC surveillance.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24341","type":"journal-article","created":{"date-parts":[[2022,3,25]],"date-time":"2022-03-25T21:15:32Z","timestamp":1648242932000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":36,"title":["A combination of circulating tumor cells and CA199 improves the diagnosis of pancreatic cancer"],"prefix":"10.1002","volume":"36","author":[{"given":"Junliang","family":"Chen","sequence":"first","affiliation":[{"name":"Department of General Surgery Shengjing Hospital of China Medical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huaitao","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of General Surgery Shengjing Hospital of China Medical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of General Surgery Shengjing Hospital of China Medical University  Shenyang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhihao","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of General Surgery Shengjing Hospital of China Medical University  Shenyang 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