{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T07:16:36Z","timestamp":1785568596081,"version":"3.56.0"},"reference-count":30,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,3,15]],"date-time":"2022-03-15T00:00:00Z","timestamp":1647302400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Extensive and inappropriate use of quinolones has led to growing resistance rates to these broad\u2010spectrum antibiotics. The present study purposed to investigate the prevalence of plasmid\u2010mediated quinolone resistance (PMQR) genes in <jats:italic>Klebsiella pneumoniae<\/jats:italic> clinical isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Ninety\u2010two non\u2010repetitive <jats:italic>K.\u00a0pneumoniae<\/jats:italic> clinical isolates were confirmed by standard microbiological methods. Antibacterial susceptibility of isolates toward seven agents from the quinolone family was evaluated by the disc diffusion method. Ciprofloxacin minimum inhibitory concentrations (MICs) were determined using the standard agar dilution method. PCR amplification was used to detect the existence of PMQR genes in the studied isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the present study, significant quinolones' resistance (40%) was observed in <jats:italic>K.\u00a0pneumoniae<\/jats:italic> isolates, and most of the strains were resistant to nalidixic acid (94.6%) and ofloxacin (45.6%). MIC analysis showed 15\u00a0strains were resistant to 6\u2013128\u00a0\u03bcg\/ml of ciprofloxacin, and five were intermediately\u2010resistant. PMQR genes were detected in 88% of all isolates. <jats:italic>Acc(6\u2019)\u2010Ib\u2010cr<\/jats:italic> was constituted half of the total PMQR genes detected among ciprofloxacin non\u2010susceptible isolates. Of 20 ciprofloxacin non\u2010susceptible isolates, 65% (n\u00a0=\u00a013) harbored multiple PMQR determinants, and 15\u00a0strains were determined as integron carriage.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The findings of this study indicated considerable resistance against quinolones, which could be correlated with the extensive and inappropriate use of this class of antibiotics as empirical treatment.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24342","type":"journal-article","created":{"date-parts":[[2022,3,16]],"date-time":"2022-03-16T04:29:32Z","timestamp":1647404972000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":21,"title":["Investigation of plasmid\u2010mediated quinolone resistance genes among clinical isolates of <i>Klebsiella pneumoniae<\/i> in southwest Iran"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6379-3827","authenticated-orcid":false,"given":"Nabi","family":"Jomehzadeh","sequence":"first","affiliation":[{"name":"Department of Microbiology School of Medicine Abadan University of Medical Sciences  Abadan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Khadijeh","family":"Ahmadi","sequence":"additional","affiliation":[{"name":"Department of Microbiology School of Medicine Abadan University of Medical Sciences  Abadan Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mozhdeh Amiri","family":"Bahmanshiri","sequence":"additional","affiliation":[{"name":"Department of Microbiology School of Medicine Abadan University of Medical Sciences  Abadan 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