{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,17]],"date-time":"2026-07-17T12:12:31Z","timestamp":1784290351152,"version":"3.55.0"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T00:00:00Z","timestamp":1647820800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>We explored the therapeutic effects of Adipose\u2010derived mesenchymal stem cells (ADMSCs) and Synovial\u2010derived mesenchymal stem cells (SDMSCs) on osteoarthritis (OA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>SDMSCs and ADMSCs were co\u2010cultured with chondrocytes and stimulated with interleukin (IL)\u20101\u03b2. An OA model was established on rats by intra\u2010articular injection with ADMSCs and SDMSCs. After 8\u00a0weeks, the joint diameter difference was detected, and histological staining was used to observe the pathological changes in cartilage tissue. Enzyme\u2010linked immunosorbent assay (ELISA) was used to detect the expressions of IL\u20106, tumor necrosis factor (TNF)\u2010\u03b1 and IL\u20101\u03b2 in joint fluid. The expressions of COL2A1, Aggrecan, Matrix metalloproteinase (MMP)\u201013, SOX9, IL\u20106, TNF\u2010\u03b1 and IL\u20101\u03b2 were detected by qRT\u2010PCR and Western blotting in cartilage tissue. Reactive oxygen species (ROS) content in cells and cartilage tissues was detected by ROS kit.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>SDMSCs and ADMSCs co\u2010cultured with chondrocytes could reduce MMP\u201013 expression, increase the expressions of COL2A1, Aggrecan and SOX9, as well as reverse the effects of IL\u20101\u03b2 on promoting ROS content and inflammatory factors levels. After the OA model was established, the injection of ADMSCs and SDMSCs reduced the differences in joint diameter and tissue lesions in OA rats. The OA model led to increased levels of IL\u20106, TNF\u2010\u03b1 and IL\u20101\u03b2 in joint fluid and cartilage tissue, while the injection of ADMSCs and SDMSCs inhibited the inflammatory factor levels in OA rats, and increased the expressions of COL2A1, Aggrecan and SOX9 in OA rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>ADMSCs and SDMSCs improve osteoarthritis in rats by reducing chondrocyte ROS and inhibiting inflammatory response.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24353","type":"journal-article","created":{"date-parts":[[2022,3,21]],"date-time":"2022-03-21T11:37:51Z","timestamp":1647862671000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Human adipose and synovial mesenchymal stem cells improve osteoarthritis in rats by reducing chondrocyte reactive oxygen species and inhibiting inflammatory 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