{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,6]],"date-time":"2026-03-06T08:09:47Z","timestamp":1772784587638,"version":"3.50.1"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,3,25]],"date-time":"2022-03-25T00:00:00Z","timestamp":1648166400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Vascular cell adhesion molecule (VCAM\u20101) mediates pulpitis via regulating interleukin (IL)\u20101\u03b2. microRNA (<jats:italic>miR)<\/jats:italic>\u2010<jats:italic>126<\/jats:italic> was reported to regulate the VCAM\u20101 under many different pathophysiological circumstances. We investigated variations of <jats:italic>miR<\/jats:italic>\u2010<jats:italic>126<\/jats:italic> and VCAM\u20101 in inflamed patient pulp tissues and determined potential roles of <jats:italic>miR<\/jats:italic>\u2010<jats:italic>126<\/jats:italic> in pulpitis using human dental pulp cells (hDPCs) in vitro.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We quantitatively measured the transcripts of <jats:italic>miR<\/jats:italic>\u2010<jats:italic>126<\/jats:italic> and VCAM\u20101 in inflamed human pulp tissues using qRT\u2010PCR and compared with those from healthy human pulp tissues. In addition, we transfected <jats:italic>miR<\/jats:italic>\u2010<jats:italic>126<\/jats:italic> in hDPCs using plasmid DNA (pDNA)\u2010encoding <jats:italic>miR<\/jats:italic>\u2010<jats:italic>126<\/jats:italic> delivered by polyethylenimine (PEI) nanoparticles.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The irreversible pulpitis significantly reduced <jats:italic>miR<\/jats:italic>\u2010<jats:italic>126<\/jats:italic> and increased the transcript of VCAM\u20101 in pulp tissues (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). pDNA\u2010encoding <jats:italic>miR<\/jats:italic>\u2010<jats:italic>126<\/jats:italic> delivered PEI nanoparticles and effectively upregulated the expression of <jats:italic>miR<\/jats:italic>\u2010<jats:italic>126<\/jats:italic> in hDPCs (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05). The overexpression of <jats:italic>miR<\/jats:italic>\u2010<jats:italic>126<\/jats:italic> could effectively suppress the transcripts and protein levels of VCAM\u20101 and IL\u20101\u03b2 induced by Pg\u2010LPS at 100ng\/mL in DPCs (<jats:italic>p<\/jats:italic>\u00a0&lt;\u00a00.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:italic>miR<\/jats:italic>\u2010<jats:italic>126<\/jats:italic> is involved in pulpitis and downregulated the VCAM\u20101 and IL\u20101\u03b2 in DPCs. <jats:italic>miR<\/jats:italic>\u2010<jats:italic>126<\/jats:italic>\u00a0may be a potential target to attenuate the inflammation of pulpitis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24371","type":"journal-article","created":{"date-parts":[[2022,3,25]],"date-time":"2022-03-25T21:16:21Z","timestamp":1648242981000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<i>microRNA\u2010126<\/i> inhibits vascular cell adhesion molecule\u20101 and interleukin\u20101beta in human dental pulp cells"],"prefix":"10.1002","volume":"36","author":[{"given":"Long","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of General Dentistry Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine College of Stomatology Shanghai Jiao Tong University National Center for Stomatology National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology  Shanghai China"},{"name":"Iowa Institute for Oral Health Research College of Dentistry The University of Iowa  Iowa City Iowa USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tadkamol","family":"Krongbaramee","sequence":"additional","affiliation":[{"name":"Iowa Institute for Oral Health Research College of Dentistry The University of Iowa  Iowa City Iowa USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinhai","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of General Dentistry Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine College of Stomatology Shanghai Jiao Tong University National Center for Stomatology National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Zhu","sequence":"additional","affiliation":[{"name":"Iowa Institute for Oral Health Research College of Dentistry The University of Iowa  Iowa City Iowa USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yaqin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of General Dentistry Shanghai Ninth People\u2019s Hospital Shanghai Jiao Tong University School of Medicine College of Stomatology Shanghai Jiao Tong University National Center for Stomatology National Clinical Research Center for Oral Diseases Shanghai Key Laboratory of Stomatology  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3782-6450","authenticated-orcid":false,"given":"Liu","family":"Hong","sequence":"additional","affiliation":[{"name":"Iowa Institute for Oral Health Research College of Dentistry The University of Iowa  Iowa City Iowa 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