{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T13:43:25Z","timestamp":1788443005898,"version":"build-2803163510"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2022,5,6]],"date-time":"2022-05-06T00:00:00Z","timestamp":1651795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To identify differentially expressed lncRNA, miRNA, and mRNA during the pathogenesis of gout, explore the ceRNA network regulatory mechanism of gout, and seek potential therapeutic targets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>First, gout\u2010related chips were retrieved by GEO database. Then, the analysis of differentially expressed lncRNAs and mRNAs was conducted by R language and other software. Besides, miRNA and its regulated mRNA were predicted based on public databases, the intersection of differentially expressed mRNA and predicated mRNA was taken, and the lncRNA\u2010miRNA\u2010mRNA regulatory relationships were obtained to construct the ceRNA regulatory network. Subsequently, hub genes were screened by the STRING database and Cytoscape software. Then the DAVID database was used to illustrate the gene functions and related pathways of hub genes and to mine key ceRNA networks.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Three hundred and eighty\u2010eight lncRNAs and 758\u00a0mRNAs were identified with significant differential expression in gout patient, which regulates hub genes in the ceRNA network, such as JUN, FOS, PTGS2, NR4A2, and TNFAIP3. In the ceRNA network, lncRNA competes with mRNA for miRNA, thus affecting the IL\u201017\u00a0signaling pathway, TNF signaling pathway, Oxytocin signaling pathway, and NF\u2010\u03baB signaling pathway through regulating the cell's response to chemical stress. The research indicates that five miRNAs (miR\u2010429, miR\u2010137, miR\u2010139\u20105p, miR\u2010217, miR\u201023b\u20103p) and five lncRNAs (SNHG1, FAM182A, SPAG5\u2010AS1, HNF1A\u2010AS1, UCA1) play an important role in the formation and development of gout.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The interaction in the ceRNA network can affect the formation and development of gout by regulating the body's inflammatory response as well as proliferation, differentiation, and apoptosis of chondrocytes and osteoclasts. The identification of potential therapeutic targets and signaling pathways through ceRNA network can provide a reference for further research on the pathogenesis of gout.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24451","type":"journal-article","created":{"date-parts":[[2022,5,7]],"date-time":"2022-05-07T05:39:22Z","timestamp":1651901962000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Construction of lncRNA\u2010miRNA\u2010mRNA network based on ceRNA mechanism reveals the function of lncRNA in the pathogenesis of gout"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5979-259X","authenticated-orcid":false,"given":"Feng","family":"Chen","sequence":"first","affiliation":[{"name":"Ruikang Hospital Affiliated to Guangxi University of Chinese Medicine  Nanning 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