{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:46:59Z","timestamp":1786978019264,"version":"build-2736575974"},"reference-count":19,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T00:00:00Z","timestamp":1653609600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Introduction<\/jats:title>\n                    <jats:p>Rapid and accurate pathogen identification is essential for the treatment of pneumonia. Metagenomic next\u2010generation sequencing (mNGS) is a newly developed technology to obtain microbial nucleic acid sequence information quickly, efficiently, and without bias.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We performed shotgun metagenomic next\u2010generation sequencing (mNGS) of bronchoalveolar lavage fluid (BALF) for pathogen identification in pneumonia in a prospective study with 138 patients from a single center. We compared the results of mNGS with standard methods including culture, staining, and targeted PCR and evaluated the clinical applicability of mNGS.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Most of the patients (128\/138, 92.75%) were cured or improved. One patient (1\/138, 0.72%) died because of acute gastrointestinal bleeding, and 9 patients (9\/138, 6.52%) showed no improvement. mNGS identified more bacteria (53 versus 27), fewer fungi (8 versus 31), and more viruses (16 versus 1) than standard methods. In total, treatment in 34 out of 138 cases (24.64%) was adjusted and optimized because of mNGS results. Positive mNGS results contributed to a definitive diagnosis in 23 cases (16.67%), which helped guide treatment decision by either adjusting the antibiotics without de\u2010escalation or continuing the empirical treatment. mNGS also confirmed no active infection in 11 cases (7.97%) allowed for antibiotic de\u2010escalation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This prospective clinical study evaluated the clinical utility of mNGS for the diagnosis of pneumonia and showed that mNGS of BALF provides valuable information for effective treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.24498","type":"journal-article","created":{"date-parts":[[2022,5,27]],"date-time":"2022-05-27T14:08:32Z","timestamp":1653660512000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Clinical application of metagenomic next\u2010generation sequencing technology in the diagnosis and treatment of pulmonary infection pathogens: A prospective single\u2010center study of 138 patients"],"prefix":"10.1002","volume":"36","author":[{"given":"Na","family":"Li","sequence":"first","affiliation":[{"name":"Department of Respiratory Medicine The First Hospital of Jiaxing (the Affiliated Hospital of Jiaxing University)  Jiaxing Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolong","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine The First Hospital of Jiaxing (the Affiliated Hospital of Jiaxing University)  Jiaxing Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaqi","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine The First Hospital of Jiaxing (the Affiliated Hospital of Jiaxing University)  Jiaxing Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jingjing","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine The First Hospital of Jiaxing (the Affiliated Hospital of Jiaxing University)  Jiaxing Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Gu","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine The First Hospital of Jiaxing (the Affiliated Hospital of Jiaxing University)  Jiaxing Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunyuan","family":"Fei","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine The First Hospital of Jiaxing (the Affiliated Hospital of Jiaxing University)  Jiaxing Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linfeng","family":"Cao","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine The First Hospital of Jiaxing (the Affiliated Hospital of Jiaxing University)  Jiaxing Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qi","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine The First Hospital of Jiaxing (the Affiliated Hospital of Jiaxing University)  Jiaxing Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7314-4409","authenticated-orcid":false,"given":"Feng","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Respiratory Medicine The First Hospital of Jiaxing (the Affiliated Hospital of Jiaxing University)  Jiaxing Zhejiang 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utilization of metagenomic sequencing in the diagnosis and treatment monitoring of an invasive adenovirus B55 infection and subsequent herpes simplex virus encephalitis in an immunocompetent young adult. Open forum","volume":"5","author":"Fang X","year":"2018","journal-title":"Infect Dis"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cix881"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bdq.2017.10.002"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201703-0537LE"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.201706-1097LE"},{"issue":"574","key":"e_1_2_9_18_1","first-page":"e1","article-title":"Untargeted next\u2010generation sequencing\u2010based first\u2010line diagnosis of infection in immunocompromised adults: a multicentre, blinded, prospective study","volume":"23","author":"Parize P","year":"2017","journal-title":"Clin Microbiol Infect"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1128\/mBio.01888-15"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00253-018-9209-9"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.24498","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.24498","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.24498","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T22:10:48Z","timestamp":1692742248000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.24498"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,5,27]]},"references-count":19,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2022,7]]}},"alternative-id":["10.1002\/jcla.24498"],"URL":"https:\/\/doi.org\/10.1002\/jcla.24498","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,5,27]]},"assertion":[{"value":"2022-02-11","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-03","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-05-27","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e24498"}}