{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T03:44:34Z","timestamp":1787802274858,"version":"build-2784847793"},"reference-count":31,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2022,6,13]],"date-time":"2022-06-13T00:00:00Z","timestamp":1655078400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Mast cells regulate the process of preeclampsia (PE). Since we previously identified mast cells specifically expressing miR\u2010181a\u20105p in the placenta of PE patients, it is plausible to examine the effect and mechanism of mast cell\u2010derived exosomal miR\u2010181a\u20105p on trophoblast cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The miR\u2010181a\u20105p and YY1 levels were determined by quantitative real\u2010time reverse transcription\u2010polymerase chain reaction. Exosomes were identified by transmission electron microscopy, Western blot, and PKH\u201026 labeling. Mast cells or trophoblast cell malignant phenotype were detected using 3\u2010(4,5\u2010dimethyl\u20102\u2010thiazolyl)\u20102,5\u2010diphenyl\u20102\u2010H\u2010tetrazolium bromide, wound healing, and Transwell assays. Quantification of YY1 and metastasis\u2010related proteins was performed using Western blot. TargetScan, JASPAR, dual\u2010luciferase reporter genes, and chromatin immunoprecipitation were exploited to verify the relationship between miR\u2010181a\u20105p, YY1, and MMP\u20109.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>MiR\u2010181a\u20105p was overexpressed in mast cells of PE patients. Overexpressed miR\u2010181a\u20105p restrained mast cell viability. Mast cell exosomes were successfully isolated, containing high expressions of CD63 and HSP70 and low expression of Calnexin and could be transported to the cytoplasm of trophoblast cells. Mast cell exosomes attenuated the viability, migration, and invasion of HTR\u20108\/SVneo cells, inhibited YY1, N\u2010cadherin, Vimentin, and MMP\u20109 protein expressions, and promoted E\u2010cadherin protein expression. The effect of exosomes was enhanced by miR\u2010181a\u20105p mimic but was reversed by miR\u2010181a\u20105p inhibitor. MiR\u2010181a\u20105p targeted YY1 which bound to the MMP\u20109 promoter. Overexpressed YY1 in HTR\u20108\/SVneo cells accelerated the malignant phenotype of the cells and reversed the regulatory effects of exosomal miR\u2010181a\u20105p.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Mast cell\u2010derived exosomal miR\u2010181a\u20105p modulates HTR\u20108\/SVneo cell viability, migration, and invasion via YY1\/MMP\u20109.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24549","type":"journal-article","created":{"date-parts":[[2022,6,14]],"date-time":"2022-06-14T04:49:07Z","timestamp":1655182147000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Mast cell\u2010derived exosomal <scp>miR<\/scp>\u2010181a\u20105p modulated trophoblast cell viability, migration, and invasion via <scp>YY1<\/scp>\/<scp>MMP<\/scp>\u20109 axis"],"prefix":"10.1002","volume":"36","author":[{"given":"Yinfen","family":"Wang","sequence":"first","affiliation":[{"name":"Maternity Department Ningbo Women &amp; Children's Hospital  Ningbo City Zhejiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7360-6834","authenticated-orcid":false,"given":"Aner","family":"Chen","sequence":"additional","affiliation":[{"name":"Maternity Department Ningbo Women &amp; Children's Hospital  Ningbo City Zhejiang Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,6,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.118.313276"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.siny.2020.101123"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.113.01892"},{"key":"e_1_2_8_5_1","first-page":"4808072","article-title":"The roles of uterine natural killer (NK) cells and KIR\/HLA\u2010C combination in the development of preeclampsia: a systematic 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