{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T09:56:31Z","timestamp":1753869391269,"version":"3.41.2"},"reference-count":28,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,6,26]],"date-time":"2022-06-26T00:00:00Z","timestamp":1656201600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Transient receptor potential channel 1 (TRPC1) promotes tumor growth and metastasis in endometrial carcinoma (EC) cell lines, whereas its clinical role in EC management remains unclear. Therefore, this study aimed to investigate the association of TRPC1 protein expression with the clinical features and survival of EC patients, then was further validated by TRPC1 mRNA measurement and data from The Human Protein Atlas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>TRPC1 protein expression in tumor tissues and normal endometria of 176 resectable EC patients was determined using immunohistochemistry. Besides, TRPC1 mRNA expression of partial patients (<jats:italic>n<\/jats:italic>\u00a0=\u00a080) was detected using RT\u2010qPCR. Additionally, survival data from The Human Protein Atlas (derived from The Cancer Genome Atlas [TCGA]) was analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>TRPC1 protein expression was up\u2010regulated in tumor tissue compared with normal endometrium (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001). Up\u2010regulated TRPC1 protein expression was associated with stromal cervical invasion (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.044), lymphovascular invasion (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.032), and increased federation of gynecology and obstetrics (FIGO) stage (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.005). Tumor TRPC1 protein high was linked with shortened accumulating disease\u2010free survival (DFS) (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.009) and overall survival (OS) (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.026), which were also confirmed by multivariate Cox's regression analysis (both <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.050). Further, TRPC1 mRNA validation disclosed that TRPC1 mRNA high was related to shortened accumulating DFS (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.038) and exhibited a correlating trend with declined OS (lacked statistical significance) (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.162). Meanwhile, survival analysis on the data from The Human Protein Atlas (derived from TCGA) also exhibited that TRPC1 mRNA high was correlated with reduced accumulating OS (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings support TRPC1 as a prognostic biomarker in resectable EC patients.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24560","type":"journal-article","created":{"date-parts":[[2022,6,27]],"date-time":"2022-06-27T05:03:48Z","timestamp":1656306228000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Aberrant TRPC1 expression reflects stromal cervical invasion, lymphovascular invasion, elevated FIGO stage, and poor survival in resectable endometrial carcinoma patients"],"prefix":"10.1002","volume":"36","author":[{"given":"Yi","family":"Wang","sequence":"first","affiliation":[{"name":"Gynecology Department, The Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science and Technology  Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8630-355X","authenticated-orcid":false,"given":"Chun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Gynecology Department, The Central Hospital of Wuhan, Tongji Medical College Huazhong University of Science and Technology  Wuhan 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