{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:45:36Z","timestamp":1786977936842,"version":"build-2736575974"},"reference-count":50,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,7,2]],"date-time":"2022-07-02T00:00:00Z","timestamp":1656720000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Serous ovarian carcinoma (SOC) is a common malignant tumor in female reproductive system. Long noncoding RNA (lncRNA) LIFR\u2010AS1 is a tumor suppressor gene in colorectal cancer, but its effect and underlying mechanism in SOC are still unclear. Therefore, this study focuses on unveiling the regulatory mechanism of LIFR\u2010AS1 in SOC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The relationship between LIFR\u2010AS1 expression and prognosis of SOC patients was analyzed by TCGA database and Starbase, and then, the LIFR\u2010AS1 expression in SOC tissues and cells was detected by quantitative real\u2010time PCR (qRT\u2010PCR) and in situ hybridization (ISH). Besides, the relationship between LIFR\u2010AS1 and clinical characteristics was analyzed. Also, the effects of LIFR\u2010AS1 on the biological behaviors of SOC cells were measured by Cell Counting Kit\u20108, colony formation, and wound\u2010healing and Transwell assays, respectively. Western blot and qRT\u2010PCR were employed to determine the protein expressions of genes related to proliferation (PCNA), apoptosis (cleaved caspase\u20103), epithelial\u2010mesenchymal transition (E\u2010cadherin, N\u2010cadherin, and Snail).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>LIFR\u2010AS1 was lowly expressed in SOC, which was correlated with the poor prognosis of SOC patients. Low expression of LIFR\u2010AS1 in SOC was associated with the tumor size, clinical stage, lymph node metastasis, and distant metastasis. LIFR\u2010AS1 overexpression promoted the expressions of cleaved caspase\u20103 and E\u2010cadherin while suppressing the malignant behaviors (proliferation, migration, and invasion) of SOC cells, the expressions of PCNA, N\u2010cadherin, and Snail. Besides, silencing LIFR\u2010AS1 exerted the effects opposite to overexpressed LIFR\u2010AS1.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>LIFR\u2010AS1 overexpression inhibits biological behaviors of SOC cells, which may be a new therapeutic method.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.24570","type":"journal-article","created":{"date-parts":[[2022,7,2]],"date-time":"2022-07-02T05:10:21Z","timestamp":1656738621000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<scp>LncRNA LIFR\u2010AS1<\/scp>\n                    overexpression suppressed the progression of serous ovarian 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