{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,14]],"date-time":"2026-09-14T18:50:55Z","timestamp":1789411855538,"version":"build-2803163510"},"reference-count":51,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,7,9]],"date-time":"2022-07-09T00:00:00Z","timestamp":1657324800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Immune thrombocytopenia purpura (ITP) is an autoimmune disease that leads to accelerated platelet clearance. The objective of this study was to examine the clinical role of cytokines in ITP patients and to correlate them with disease stages.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>A total of 110 ITP patients were enrolled, including 55 with active ITP, 55 with remission ITP, and 55 with healthy controls. The enzyme\u2010linked immunosorbent assay technique was used to examine <jats:italic>IL<\/jats:italic>\u201010 and <jats:italic>IL<\/jats:italic>\u201022 serum levels in all subjects. Real\u2010time quantitative PCR was used to assess the mRNA expression of <jats:italic>IL<\/jats:italic>\u201010 and <jats:italic>IL<\/jats:italic>\u201022 in PBMC. The clinical significance of both cytokines was assessed using ROC analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>IL<\/jats:italic>\u201010 serum levels in active ITP patients were significantly lower than in control and remission ITP subjects (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). <jats:italic>IL<\/jats:italic>\u201022 serum levels were elevated in active ITP patients compared to the control and remission group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). mRNA expressions of <jats:italic>IL<\/jats:italic>\u201010 and <jats:italic>IL<\/jats:italic>\u201022 in active ITP patients were also having a significant difference from than control and remission ITP group (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). ROC analysis showed that <jats:italic>IL<\/jats:italic>\u201010 and <jats:italic>IL<\/jats:italic>\u201022 can differentiate the ITP patients from controls. A positive correlation between serum <jats:italic>IL<\/jats:italic>\u201010 and PBMC <jats:italic>IL<\/jats:italic>\u201010 with statistical significance was observed. Similarly, the serum <jats:italic>IL<\/jats:italic>\u201022 and PBMC <jats:italic>IL<\/jats:italic>\u201022 were correlated positively with statistical significance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>IL<\/jats:italic>\u201010 and <jats:italic>IL<\/jats:italic>\u201022 seem to predict the clinical course of ITP, as a significant imbalance of these cytokines was detected in active ITP patients.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24573","type":"journal-article","created":{"date-parts":[[2022,7,9]],"date-time":"2022-07-09T07:13:49Z","timestamp":1657350829000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Role of IL\u201010 and IL\u201022 cytokines in patients with primary immune thrombocytopenia and their clinical 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