{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,17]],"date-time":"2026-01-17T07:29:56Z","timestamp":1768634996911,"version":"3.49.0"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,7,2]],"date-time":"2022-07-02T00:00:00Z","timestamp":1656720000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Eighteen\u2010hydroxycortisol (18\u2010OHF) is a potential biomarker for differential diagnosis of the two major primary aldosteronism subtypes, aldosterone\u2010producing adenoma, and idiopathic hyperaldosteronism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Urine samples were processed, and the 18\u2010OHF in urine samples were successfully quantified by in\u2010house established dilute\u2010and\u2010shoot liquid chromatography\u2013tandem mass spectrometry (LC\u2013MS\/MS) method. Separation was accomplished on a Sigma Ascentis Express C18 column with a gradient mixture of phase (A) 0.2% formic acid in water and phase (B) 0.2% formic acid in methanol at a flow rate of 0.4\u00a0ml\/min. Mass spectrometric detection was performed in positive electrospray ionization mode via a mass spectrometer.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The linearity of urinary 18\u2010OHF ranged from 4.28 to 8.77\u2009\u00d7\u200910<jats:sup>3<\/jats:sup>\u2009nmol\/L, with a lower limit of quantification at 4.28\u2009nmol\/L. The intra\u2010 and inter\u2010precision were both below 3%. The range of analytical recovery was 97.8%\u2013109.2%. The validated dilute\u2010and\u2010shoot LC\u2013MS\/MS method was compared with the SPE LC\u2013MS\/MS method modified from the one reported in 2013. The results by Passing\u2013Bablok regression analysis and Bland\u2013Altman plotting demonstrated a good agreement between the two methods. The presented method was then applied to establish sex\u2010specific reference intervals from 62 males and 62 females, respectively. The calculated 2.5%\u201397.5% reference intervals for 24\u2010h urinary 18\u2010OHF were 113\u2013703\u2009nmol\/day for males and 71.2\u2013450\u2009nmol\/day for females.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The presented dilute\u2010and\u2010shoot LC\u2013MS\/MS method for 18\u2010OHF quantification showed a good performance in the clinical application. Furthermore, the sex\u2010specific reference intervals for 24\u2010h urinary 18\u2010OHF were first established and quite important for its application in primary aldosteronism subtyping.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24580","type":"journal-article","created":{"date-parts":[[2022,7,2]],"date-time":"2022-07-02T09:09:55Z","timestamp":1656752995000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["A dilute\u2010and\u2010shoot liquid chromatography\u2013tandem mass spectrometry method for urinary 18\u2010hydroxycortisol quantification and its application in establishing reference intervals"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8514-3358","authenticated-orcid":false,"given":"Beibei","family":"Zhao","sequence":"first","affiliation":[{"name":"Clinical Mass Spectrometry Center Guangzhou KingMed Center for Clinical Laboratory Co.,Ltd  Guangzhou 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