{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,4]],"date-time":"2026-11-04T19:51:52Z","timestamp":1793821912535,"version":"4.6.1"},"reference-count":28,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T00:00:00Z","timestamp":1657584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Dasatinib, nilotinib, and bosutinib, second\u2010generation tyrosine kinase inhibitors (TKIs), and ponatinib, a third\u2010generation TKI, are approved pharmaceuticals used in the treatment of chronic myeloid leukemia (CML). Although liquid chromatography\u2010tandem mass spectrometry assays for simultaneous quantification of the four TKIs in human serum have been reported in the literature, a high\u2010performance liquid chromatography (HPLC) assay that simultaneously quantifies these compounds has not yet been developed. This study aims to establish and validate an efficient HPLC analytical method using a photodiode array (PDA) detector for the simultaneous quantification of the four TKIs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Calibration standards were prepared by serial dilution of serum samples containing the four TKIs, followed by solid\u2010phase extraction. The four TKIs were eluted in order within 10\u00a0min using a binary HPLC gradient system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The calibration ranges were 2\u2013500\u2009ng\/ml for dasatinib, 100\u20135000\u2009ng\/ml for nilotinib, and 10\u2013500\u2009ng\/ml for bosutinib and ponatinib. Intra\u2010day and inter\u2010day precision and accuracy values were found to be in accordance with the U.S. Food and Drug Administration guidelines. The recovery rates were 92.9%\u201396.0%, 80.7%\u201386.1%, 91.6%\u201399.0%, and 86.4%\u201392.6% for dasatinib, nilotinib, bosutinib, and ponatinib, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>To the best of our knowledge, this is the first report of an HPLC\u2010PDA analytical method that allows efficient simultaneous quantification of the four TKIs in the serum of patients with CML. We believe that the method developed herein can improve the efficiency of therapeutic drug monitoring in patients with CML in clinical practice.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24598","type":"journal-article","created":{"date-parts":[[2022,7,12]],"date-time":"2022-07-12T11:17:16Z","timestamp":1657624636000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Simultaneous quantification of dasatinib, nilotinib, bosutinib, and ponatinib using high\u2010performance liquid chromatography\u2013Photodiode array detection"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9359-9945","authenticated-orcid":false,"given":"Yuta","family":"Yokoyama","sequence":"first","affiliation":[{"name":"Division of Pharmaceutical Care Sciences, Center for Social Pharmacy and Pharmaceutical Care Sciences Keio University Faculty of Pharmacy  Tokyo Japan"},{"name":"Division of Pharmaceutical Care 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