{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,10]],"date-time":"2026-10-10T08:13:21Z","timestamp":1791620001259,"version":"4.3.4"},"reference-count":38,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,7,23]],"date-time":"2022-07-23T00:00:00Z","timestamp":1658534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Urinary tract infections (UTIs) and bacterial resistance to antibiotics is global health problem and a threat to public health in many countries.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Aims<\/jats:title>\n                    <jats:p>\n                      The study aimed to determine the prevalence of MDR\n                      <jats:italic>Escherichia coli<\/jats:italic>\n                      and\n                      <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                      in UTI patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials &amp; methods<\/jats:title>\n                    <jats:p>The midstream urine samples of 120 patients were collected and cultured as described by the protocols at the respective sample collection sites on MacConkey Blood agar. Samples were tested by using the fully automated VITEK 2 Compact system for Gram\u2010negative identification and detection of antimicrobial susceptibility of microorganisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      The most prevalent pathogen was\n                      <jats:italic>E. coli<\/jats:italic>\n                      , which was found in 82 (68.3%) urine samples, followed by\n                      <jats:italic>K. pneumonia<\/jats:italic>\n                      , found in 38 (31.7%) urine samples. As far as antibiotic resistance is concerned,\n                      <jats:italic>E. coli<\/jats:italic>\n                      isolates were found to be highly resistant for ceftriaxone (89.0% of the isolates), ampicillin (86.6%), levofloxacin (82.9%), cefotaxime (79.3%), aztreonam (74.4%), ceftazidime (68.3%) and gentamicin, piperacillin, and trimethoprim\u2010sulfamethoxazole, 54.9 and 53.7%, respectively. The\n                      <jats:italic>E. coli<\/jats:italic>\n                      isolates were found to be relatively less resistant to imipenem (2.4%), cefepime (34.1%), and ciprofloxacin (35.4%). For\n                      <jats:italic>K. pneumonia<\/jats:italic>\n                      isolates, high resistance rates were observed for piperacillin (81.6%), levofloxacin (78.9%), ampicillin (76.3%), cefotaxime (73.7%), trimethoprim\u2010sulfamethoxazole (71.1%), ceftazidime (65.8%), gentamicin (63.2%), cefepime (50.0%), and aztreonam (44.7%). However, moderate resistance rates were detected for these were found to be less resistant for imipenem (13.2%), ceftriaxone (31.6%), and ciprofloxacin (36.8%).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      <jats:italic>E. coli<\/jats:italic>\n                      and\n                      <jats:italic>K. pneumoniae<\/jats:italic>\n                      from the clinical isolates displayed high resistance to many antibiotics in UTI patients.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.24619","type":"journal-article","created":{"date-parts":[[2022,7,23]],"date-time":"2022-07-23T15:24:12Z","timestamp":1658589852000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":80,"title":["The prevalence of multiple drug resistance\n                    <i>Escherichia coli<\/i>\n                    and\n                    <i>Klebsiella pneumoniae<\/i>\n                    isolated from patients with urinary tract infections"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4799-0879","authenticated-orcid":false,"given":"Mays B.","family":"Jalil","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Techniques Al\u2010Kunooze University College  Basrah Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8568-1567","authenticated-orcid":false,"given":"Mohammed Younus Naji","family":"Al Atbee","sequence":"additional","affiliation":[{"name":"Department of Nephrology College of Medicine, University of Basrah  Basrah Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,7,23]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-018-2960-9"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408398.2011.625574"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2019.02.007"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.2147\/DMSO.S51792"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro3432"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2362.2008.02014.x"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciq257"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-018-4254-0"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/258517"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2017.11.061"},{"key":"e_1_2_9_12_1","volume-title":"Antimicrobial resistance surveillance in Europe 2014. Annual Report of the European Antimicrobial Resistance Surveillance Network (EARS\u2010Net)","author":"European Centre for Disease Prevention and Control","year":"2015"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMp1215093"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0255-0857(21)02884-x"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1080\/1120009X.2017.1380395"},{"key":"e_1_2_9_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/jobm.201700008"},{"key":"e_1_2_9_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2007.01724.x"},{"key":"e_1_2_9_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2010.190"},{"key":"e_1_2_9_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkm348"},{"key":"e_1_2_9_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s15010-009-8460-5"},{"key":"e_1_2_9_21_1","doi-asserted-by":"publisher","DOI":"10.3126\/jist.v23i1.22159"},{"key":"e_1_2_9_22_1","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.10553"},{"key":"e_1_2_9_23_1","unstructured":"ShariatpanahiSS.Prevalence of Enterobacteriaceae involved in urinary tract infection and antimicrobial susceptibility pattern in adult outpatients in Isfahan. Third Iran Congr Med Bacteriol Milad Hosp Tehran;2015.https:\/\/www.researchgate.net\/publication\/283794334_Prevalence_of_Enterobacteriaceae_involved_in_urinary_tract_infection_and_antimicrobial_susceptibility_pattern_in_adult_outpatients_in_Isfahan"},{"key":"e_1_2_9_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0047752"},{"key":"e_1_2_9_25_1","first-page":"264","article-title":"Prevalence, antibiotic susceptibility pattern, and production of extended\u2010spectrum beta\u2010lactamases amongst clinical isolates of Klebsiella pneumoniae at armed forces Hospital in Saudi Arabia","volume":"19","author":"Ahmad S","year":"2009","journal-title":"J Coll Physicians Surg Pak"},{"issue":"1","key":"e_1_2_9_26_1","first-page":"25","article-title":"Bacteriology of urinary tract infection among patient attending TU teaching hospital, Kathmandu, Nepal","volume":"9","author":"Kattel HP","year":"2008","journal-title":"J Nepal Assoc Med Lab Sci"},{"key":"e_1_2_9_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(08)70018-5"},{"key":"e_1_2_9_28_1","doi-asserted-by":"publisher","DOI":"10.1186\/2047-2994-1-23"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.46.8.2540-2545.2002"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-0711-7-13"},{"issue":"2","key":"e_1_2_9_31_1","first-page":"56","article-title":"Antimicrobial susceptibility pattern of urinary tract infection causing human pathogenic bacteria","volume":"3","author":"Manikandan S","year":"2011","journal-title":"Asian J Med Sci"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-016-1495-1"},{"key":"e_1_2_9_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12941-018-0255-z"},{"key":"e_1_2_9_34_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12889-019-7173-7"},{"key":"e_1_2_9_35_1","doi-asserted-by":"publisher","DOI":"10.1100\/2012\/349597"},{"issue":"4","key":"e_1_2_9_36_1","first-page":"393","article-title":"Clinical and laboratory profile of urinary tract infections associated with extended Spectrum \u03b2\u2010lactamase producing Escherichia coli and Klebsiella pneumoniae","volume":"46","author":"Al Yousef SA","year":"2016","journal-title":"Ann Clin Lab Sci"},{"key":"e_1_2_9_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eimc.2016.02.018"},{"key":"e_1_2_9_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtumed.2021.03.004"},{"key":"e_1_2_9_39_1","doi-asserted-by":"publisher","DOI":"10.1080\/00325481.2019.1680052"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.24619","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.24619","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.24619","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,21]],"date-time":"2023-08-21T23:07:32Z","timestamp":1692659252000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.24619"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,7,23]]},"references-count":38,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["10.1002\/jcla.24619"],"URL":"https:\/\/doi.org\/10.1002\/jcla.24619","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,7,23]]},"assertion":[{"value":"2022-03-01","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-12","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-23","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e24619"}}