{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,1]],"date-time":"2026-05-01T03:17:53Z","timestamp":1777605473131,"version":"3.51.4"},"reference-count":42,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T00:00:00Z","timestamp":1659657600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>LncRNAs are closely related to cutaneous melanoma (CM) tumorigenesis and metastasis, and it can affect the progression of CM by regulating cell proliferation, migration, invasion, apoptosis, and other cellular mechanisms. This study investigated the role of LINC00665 in CM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Expressions of LINC00665, miR\u2010339\u20103p, and tubulin beta chain (TUBB) in CM cells were analyzed by qRT\u2010PCR and\/or Western blot. The LINC00665\/miR\u2010339\u20103p\/TUBB targeting network was predicted by bioinformatics tools, screened out by Venn diagrams and analyzed by Pearson's correlation coefficients, followed by validation <jats:italic>via<\/jats:italic> dual\u2010luciferase reporter assay and\/or pull\u2010down assay. Transfection of siLINC00665 or miR\u2010339\u20103p inhibitor\/mimic was conducted with CM cells whose viability, proliferation, migration, invasion, cell cycle progression, and apoptosis were measured by CCK\u20108 assay, colony formation assay, wound healing assay, Transwell assay, and flow cytometry. The associations of TUBB with tumor biological characteristics and other proteins were analyzed by CanserSEA and String, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>High\u2010expressed LINC00665 was detected in CM cells. Silencing LINC00665 decreased CM cell viability; inhibited colony formation, cell cycle progression, migration and invasion; enhanced apoptosis; and upregulated miR\u2010339\u20103p. LINC00665 targeted miR\u2010339\u20103p which targeted TUBB. MiR\u2010339\u20103p upregulation induced effects similar to the LINC00665\u2010silencing\u2010induced effects and could downregulate TUBB, which was associated with malignant behaviors and related to other five proteins. MiR\u2010339\u20103p downregulation induced the opposite effects of what miR\u2010339\u20103p upregulation induced, and the miR\u2010339\u20103p downregulation\u2010induced effects could be reversed by LINC00665 silencing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Silencing LINC00665 inhibits in vitro CM progression and induces apoptosis <jats:italic>via<\/jats:italic> the miR\u2010339\u20103p\/TUBB axis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24630","type":"journal-article","created":{"date-parts":[[2022,8,5]],"date-time":"2022-08-05T07:24:46Z","timestamp":1659684286000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Silencing LINC00665 inhibits cutaneous melanoma in vitro progression and induces apoptosis via the miR\u2010339\u20103p\/TUBB"],"prefix":"10.1002","volume":"36","author":[{"given":"Yi","family":"Liu","sequence":"first","affiliation":[{"name":"Dermatological Department Nanxishan Hospital of Guangxi Zhuang Autonomous Region  Guilin City China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shanshan","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Dermatology &amp; STD QingDao No.8 People's Hospital  Qingdao China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qichao","family":"Ma","sequence":"additional","affiliation":[{"name":"Dermatological Department Ningbo Yinzhou No 2. Hospital  Ningbo City China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8887-4519","authenticated-orcid":false,"given":"Haigang","family":"Zhu","sequence":"additional","affiliation":[{"name":"Dermatological Department Ningbo Yinzhou No 2. Hospital  Ningbo City China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,8,5]]},"reference":[{"key":"e_1_2_8_2_1","first-page":"814396","article-title":"Sonography of the primary cutaneous melanoma: a review","volume":"2012","author":"Wortsman X","year":"2012","journal-title":"Radiol Res Pract"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21590"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2014.246"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-018-2726-1"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-14-2566"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0172498"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb.2480"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.28559"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1155\/2018\/2086564"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-018-2820-4"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.29449"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3349\/ymj.2019.60.9.842"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-1361-3"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S214588"},{"issue":"19","key":"e_1_2_8_16_1","first-page":"9852","article-title":"LINC00665 facilitates the progression of osteosarcoma via sponging miR\u20103619\u20105p","volume":"24","author":"Zhang DW","year":"2020","journal-title":"Eur Rev Med Pharmacol Sci"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3378-5_21"},{"key":"e_1_2_8_18_1","first-page":"2896","article-title":"Integrative analysis of Long noncoding RNA (lncRNA), microRNA (miRNA) and mRNA expression and construction of a competing endogenous RNA (ceRNA) network in metastatic melanoma","volume":"25","author":"Wang LX","year":"2019","journal-title":"Med Sci Moni Int Med J Exp Clin Res"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/cam4.2583"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaad.2018.08.055"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/jso.21962"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2017.43"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.05.044"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.20780"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/cpr.12457"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S241578"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/cphy.c110012"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2020.110214"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/s11658-020-00235-8"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13577-020-00393-z"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/35077213"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41419-019-2090-3"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.2147\/OTT.S241627"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2015.3661"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.3233\/CBM-190939"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-15-2932"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.omto.2019.07.004"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2018.12.001"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1369\/jhc.2008.952002"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.20436"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.12659\/MSM.924343"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1080\/01635581.2016.1134599"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.24630","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/jcla.24630","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.24630","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,22]],"date-time":"2023-08-22T03:09:07Z","timestamp":1692673747000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.24630"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,8,5]]},"references-count":42,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2022,9]]}},"alternative-id":["10.1002\/jcla.24630"],"URL":"https:\/\/doi.org\/10.1002\/jcla.24630","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,8,5]]},"assertion":[{"value":"2022-03-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-07-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2022-08-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e24630"}}