{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T18:03:19Z","timestamp":1791050599485,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,8,28]],"date-time":"2022-08-28T00:00:00Z","timestamp":1661644800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The objective was to investigate the expression of the cGAS\u2010STING pathway\u2010associated protein in idiopathic inflammatory myopathy (IIM) and to investigate whether it is related to myofiber atrophy\/necrosis in patients with dermatomyositis and immune\u2010mediated necrotizing myopathy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Material and Methods<\/jats:title>\n                    <jats:p>Muscle specimens obtained by open biopsy from 26 IIM patients (14 with dermatomyositis (DM), 8 with immune\u2010mediated necrotizing myopathy (IMNM), and 4 with other types of IIM), 4 dystrophinopathy, and 9 control patients were assessed for expression of cGAS\u2010STING pathway members via Western blot, quantitative real\u2010time PCR analysis (qRT\u2010PCR), and immunochemistry. Meanwhile, analysis its location distribution througn immunochemistry.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Compared to the control group, the expression of cGAS, STING, and related molecules was obviously increased in muscle samples of IIM patients. Upregulated cGAS and STING were mainly located in the vascular structure, inflammatory infiltrates, and atrophic and necrotic fibers. While comparing to the Dys patients, the mRNA level of cGAS, STING, and TNF\u2010a was upregulated, meanwhile, the protein of the TBK1, P\u2010TBK1, and P\u2010IRF3 associated with interferon upregulation was overexpressed through Western blot in IMNM and DM. Considering that cGAS and STING are located in necrotic and Mx1\u2010positive atrophic fibers, it is really possible that the cGAS\u2010STING pathway may lead to fibers atrophy\/necrosis by producing IFNs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The cGAS\u2010STING pathway was activated in the muscle samples of IIM patients and its activation may be the reason of myofiber atrophy and necrosis in DM and IMNM patients.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.24631","type":"journal-article","created":{"date-parts":[[2022,8,28]],"date-time":"2022-08-28T15:04:00Z","timestamp":1661699040000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Activation of\n                    <scp>cGAS\u2010STING<\/scp>\n                    pathway \u2013 A possible cause of myofiber atrophy\/necrosis in dermatomyositis and immune\u2010mediated necrotizing myopathy"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2394-6419","authenticated-orcid":false,"given":"Meichen","family":"Zhou","sequence":"first","affiliation":[{"name":"Department of Neurology First Affiliated Hospital of Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxiao","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Neurology First Affiliated Hospital of Soochow University  Suzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenhua","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Neurology Huashan hospital  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jianhua","family":"Jiang","sequence":"additional","affiliation":[{"name":"Department of Neurology First Affiliated Hospital of Soochow University 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