{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T06:42:22Z","timestamp":1768286542436,"version":"3.49.0"},"reference-count":29,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,8,10]],"date-time":"2022-08-10T00:00:00Z","timestamp":1660089600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The use of FR\u2009+\u2009CTC to distinguish lung cancer from benign lung disease has been well studied. However, the effective method to differentiate precursor glandular lesions from benign\/malignant pulmonary diseases is rare.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>380 patients with indeterminate pulmonary nodules were prospectively recruited. Peripheral blood samples were collected from all participants before surgery for analyzing FR\u2009+\u2009CTC levels. The performance of FR\u2009+\u2009CTC to identify lung precursor lesions were analyzed by receiver operating characteristic (ROC) curve.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      FR\u2009+\u2009CTC can effectively differentiate precursor from benign pulmonary diseases in all included patients (cutoff: 9.22 FU\/3\u00a0ml, AUC\u00a0=\u00a00.807, (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u20090.0001, sensitivity: 69.17%, specificity: 82.46%) and patients with single pulmonary lesion (cutoff: 9.03 FU\/3\u00a0ml, AUC\u00a0=\u00a00.842,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.0001, sensitivity: 75.20%, specificity: 83.00%). However, FR\u2009+\u2009CTC cannot differentiate precursor from benign pulmonary diseases in multiple lesions patients (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.110). FR\u2009+\u2009CTC neither differentiate precursor from malignant pulmonary lesions in all included patients (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.715), single nor multiple lesions patients (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.867,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0=\u00a00.692, respectively). Total number of pulmonary nodules, MTD, location (lower vs upper) were independent risk factors for malignancy (AOR, 95% CI: 3.104 (1.525, 6.316), 3.148 (1.722, 5.754), 2.098 (1.132, 3.888), respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Preoperative FR\u2009+\u2009CTC can be identified in precursor glandular lesions and utilized to differentiate from benign pulmonary diseases. Total number of pulmonary nodules, MTD, location (lower vs upper) were independent risk factors for malignancy.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.24654","type":"journal-article","created":{"date-parts":[[2022,8,11]],"date-time":"2022-08-11T00:19:30Z","timestamp":1660177170000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Folate receptor\u2010positive circulating tumor cells in the preoperative diagnosis of indeterminate pulmonary nodules"],"prefix":"10.1002","volume":"36","author":[{"given":"Zhixin","family":"Li","sequence":"first","affiliation":[{"name":"Department of Thoracic Surgery, Shanghai Pulmonary Hospital School of Medicine, Tongji University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jianqiao","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Shanghai Pulmonary Hospital School of Medicine, Tongji University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongqiang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery Linqu County People's hospital  Weifang China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Cai","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Shanghai Pulmonary Hospital School of Medicine, Tongji University  Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6071-0424","authenticated-orcid":false,"given":"Xiaogang","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Thoracic Surgery, Shanghai Pulmonary Hospital School of Medicine, Tongji University  Shanghai 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