{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T23:21:16Z","timestamp":1780960876758,"version":"3.54.1"},"reference-count":44,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2022,8,16]],"date-time":"2022-08-16T00:00:00Z","timestamp":1660608000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005001","name":"Ahvaz Jundishapur University of Medical Sciences","doi-asserted-by":"publisher","award":["OG\u20109948"],"award-info":[{"award-number":["OG\u20109948"]}],"id":[{"id":"10.13039\/501100005001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The most frequent species of <jats:italic>Candida<\/jats:italic> to infect and colonize patients with neutropenia is still <jats:italic>Candida albicans<\/jats:italic>. This study aimed to provide detailed information on the phenotype, genotype, and mating type of oral <jats:italic>C.\u00a0albicans<\/jats:italic> isolated from neutropenic pediatric patients, and to investigate how these characteristics are related.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Two hundred fifty\u2010four oral samples from patients under 18\u2009years old with neutropenia and malignancies were collected from January to October 2021. Samples were cultured on CHROMagar <jats:italic>Candida<\/jats:italic>. Isolates of <jats:italic>C.\u00a0albicans<\/jats:italic> were identified with the germ tube test, chlamydospore production on cornmeal agar, and PCR\u2010RFLP. Genotyping of <jats:italic>C.\u00a0albicans<\/jats:italic> isolates was carried out by amplifying the <jats:italic>25S rDNA<\/jats:italic> gene with specific CAINT\u2010L and CA\u2010INT\u2010R primers. <jats:italic>MTL<\/jats:italic>a1 and <jats:italic>MTL<\/jats:italic>\u03b11 primers were used to identify each mating type. Yeast peptone dextrose supplemented with phloxine B was used to identify different phenotypes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Ninety\u2010two (36%) patients were positive for <jats:italic>C.\u00a0albicans<\/jats:italic>. The mean age of patients was 7.85. Fifty\u2010three (58.9%) isolates demonstrated type A, 15 (16.7%) type B, 15 (16.7%) types D\/E, and 7 (7.7%) type C. Three isolates each (3.3%) were homozygous for <jats:italic>MTL<\/jats:italic>a or homozygous for <jats:italic>MTL\u03b1<\/jats:italic>. All of the <jats:italic>MTL<\/jats:italic>\u2010homozygous isolates were genotype A. There was a significant correlation between patients' underlying disease and genotype (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.036). There was a significant correlation between mating type and genotype (<jats:italic>p<\/jats:italic>\u00a0=\u00a00.000).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Most of the isolates exhibited a white phenotype, noted in the literature as the most virulent. Moreover, heterozygous strains were frequent and may play a role in <jats:italic>Candida<\/jats:italic> colonization.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24664","type":"journal-article","created":{"date-parts":[[2022,8,16]],"date-time":"2022-08-16T07:44:22Z","timestamp":1660635862000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Phenotype, genotype, and mating type determination in oral <i>Candida albicans<\/i> isolates from pediatric patients with neutropenia"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8959-6482","authenticated-orcid":false,"given":"Hadis","family":"Jafarian","sequence":"first","affiliation":[{"name":"Department of Medical Mycology, School of Medicine Ahvaz Jundishapur University of Medical Sciences  Ahvaz Iran"},{"name":"Infectious and Tropical Diseases Research Center, Health Research Institute Ahvaz Jundishapur University of Medical Sciences  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