{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:44:58Z","timestamp":1786977898913,"version":"build-2736575974"},"reference-count":42,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,8,21]],"date-time":"2022-08-21T00:00:00Z","timestamp":1661040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006402","name":"Fasa University of Medical Sciences","doi-asserted-by":"publisher","award":["99157"],"award-info":[{"award-number":["99157"]}],"id":[{"id":"10.13039\/501100006402","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>SARS\u2010CoV\u20102 is one of the most contagious viruses in the Coronaviridae (CoV) family, which has become a pandemic. The aim of this study is to understand more about the role of hsa_circ_0004812 in the SARS\u2010CoV\u20102 related cytokine storm and its associated molecular mechanisms.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>cDNA synthesis was performed after total RNA was extracted from the peripheral blood mononuclear cells (PBMC) of 46 patients with symptomatic COVID\u201019, 46 patients with asymptomatic COVID\u201019, and 46 healthy controls. The expression levels of hsa_circ_0004812, hsa\u2010miR\u20101287\u20105p, IL6R, and RIG\u2010I were determined using qRT\u2010PCR, and the potential interaction between these molecules was confirmed by bioinformatics tools and correlation analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>hsa_circ_0004812, IL6R, and RIG\u2010I are expressed higher in the severe symptom group compared with the negative control group. Also, the relative expression of these genes in the asymptomatic group is lower than in the severe symptom group. The expression level of hsa\u2010miR\u20101287\u20105p was positively correlated with symptoms in patients. The results of the bioinformatics analysis predicted the sponging effect of hsa_circ_0004812 as a competing endogenous RNA on hsa\u2010miR\u20101287\u20105p. Moreover, there was a significant positive correlation between hsa_circ_0004812, RIG\u2010I, and IL\u20106R expressions, and also a negative expression correlation between hsa_circ_0004812 and hsa\u2010miR\u20101287\u20105p and between hsa\u2010miR\u20101287\u20105p, RIG\u2010I, and IL\u20106R.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The results of this in\u2010vitro and in silico study show that hsa_circ_0004812\/hsa\u2010miR\u20101287\u20105p\/IL6R, RIG\u2010I can play an important role in the outcome of COVID\u201019.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.24666","type":"journal-article","created":{"date-parts":[[2022,8,22]],"date-time":"2022-08-22T01:44:01Z","timestamp":1661132641000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Upregulation of hsa_circ_0004812 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