{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T10:57:34Z","timestamp":1769857054631,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T00:00:00Z","timestamp":1661731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The inhibitor of \u03b2\u2010catenin and T\u2010cell factor (ICAT) is a direct negative regulator of the canonical Wnt signaling pathway, which is an attractive therapeutic target for colorectal cancer (CRC). Accumulating evidence suggests that ICAT interacts with other proteins to exert additional functions, which are not yet fully elucidated.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The overexpression of ICAT of CRC cells was conducted by lentivirus infection and plasmids transfection and verified by quantitative real\u2010time reverse transcription\u2010polymerase chain reaction (real\u2010time RT\u2010PCR) and Western blotting. The effect of ICAT on the mobility of CRC cells was assessed by wound healing assay and transwell assay in vitro and lung metastasis in vivo. New candidate ICAT\u2010interacting proteins were explored and verified using the STRING database, silver staining, co\u2010immunoprecipitation mass spectrometry analysis (Co\u2010IP\/MS), and immunofluorescence (IF) staining analysis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Result<\/jats:title>\n                    <jats:p>Inhibitor of \u03b2\u2010catenin and T\u2010cell factor overexpression promoted in vitro cell migration and invasion and tumor metastasis in vivo. Co\u2010IP\/MS analysis and STRING database analyses revealed that junction plakoglobin (JUP), a homolog of \u03b2\u2010catenin, was involved in a novel protein interaction with ICAT. Furthermore, JUP downregulation impaired ICAT\u2010induced migration and invasion of CRC cells. In addition, ICAT overexpression activated the NF\u2010\u03baB signaling pathway, which led to enhanced CRC cell migration and invasion.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Inhibitor of \u03b2\u2010catenin and T\u2010cell factor promoted CRC cell migration and invasion by interacting with JUP and the NF\u2010\u03baB signaling pathway. Thus, ICAT could be considered a protein diagnostic biomarker for predicting the metastatic ability of CRC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.24678","type":"journal-article","created":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T10:24:07Z","timestamp":1661768647000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["<scp>ICAT<\/scp>\n                    promotes colorectal cancer metastasis via binding to\n                    <scp>JUP<\/scp>\n                    and activating the\n                    <scp>NF\u2010\u03baB<\/scp>\n                    signaling pathway"],"prefix":"10.1002","volume":"36","author":[{"given":"Zihan","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory, The Sixth Affiliated Hospital Sun Yat\u2010sen University  Guangzhou China"},{"name":"Guangdong Institute of Gastroenterology  Guangzhou Guangdong 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