{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,22]],"date-time":"2026-04-22T05:05:02Z","timestamp":1776834302693,"version":"3.51.2"},"reference-count":37,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T00:00:00Z","timestamp":1669334400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LQ22H200005"],"award-info":[{"award-number":["LQ22H200005"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Lung adenocarcinoma (LUAD) is a highly malignant tumor with a very low five\u2010year survival rate. In this study, we aimed to identify differentially expressed long\u2010chain non\u2010coding RNA (lncRNAs) and mRNAs from benign and malignant pleural effusion exosomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used gene microassay and quantitative real\u2010time reverse transcription polymerase chain reaction (RT\u2010qPCR) to detect and verify differentially expressed mRNAs and lncRNAs in benign and malignant pleural effusion exosomes. Gene Ontology (GO) functional significance and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway significance enrichment analyses were performed to identify the difference in biological processes and functions between different mRNAs. We selected the lncRNA ZBED5\u2010AS1 with an upregulated differential fold of 3.003 and conducted a preliminary study on its cellular function.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Gene microassay results revealed that 177 differentially expressed lncRNAs were upregulated, and 215 were downregulated. The top 10 upregulated were FMN1, AL118505.1, LINC00452, AL109811.2, CATG00000040683.1, AC137932.1, AC008619.1, AL450344.1, AC092718.6, and ZBED5\u2010AS1. The top 10 downregulated were TEX41, G067726, JAZF1\u2010AS1, AC027328.1, AL445645.1, AL022345.4, AC008572.1, AC123777.1, AC093714.1, and PHKG1. For the mRNAs, 79 were upregulated, and 123 were notably downregulated. GO analysis revealed that the upregulated differential mRNAs were mainly involved in \u201ccellular response to acidic pH\u201d (biological processes), \u201cendoplasmic reticulum part\u201d (cellular components), and \u201cat DNA binding, cyclase activity\u201d (molecular functions). KEGG pathways were found to be related to <jats:italic>V. cholerae<\/jats:italic> infection, Parkinson's disease, and cell adhesion molecules. RT\u2010qPCR showed that ZBED5\u2010AS1 was highly expressed in LUAD tissues, cells, and benign and malignant pleural fluid exosomes. Overexpression of ZBED5\u2010AS1 could significantly promote the proliferation, migration, invasion, and colony formation of LUAD cells, and knockdown had the opposite consequence.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The pleural effusion exosomes from patients with LUAD include several improperly expressed genes, and lncRNA\u2010ZBED5\u2010AS1 is a new biomarker that aids in our understanding of the occurrence and progression of LUAD.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24777","type":"journal-article","created":{"date-parts":[[2022,11,25]],"date-time":"2022-11-25T14:10:56Z","timestamp":1669385456000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Construction and analysis of expression profile of exosomal <scp>lncRNAs<\/scp> in pleural effusion in lung adenocarcinoma"],"prefix":"10.1002","volume":"36","author":[{"given":"Xiaolu","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine The First Affiliated Hospital of Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huixin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine The First Affiliated Hospital of Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine The First Affiliated Hospital of Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3666-1110","authenticated-orcid":false,"given":"Wenjing","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine The First 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