{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T15:01:56Z","timestamp":1780066916263,"version":"3.54.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,1]],"date-time":"2022-12-01T00:00:00Z","timestamp":1669852800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Long noncoding RNAs (lncRNAs) can be used as competitive endogenous RNAs (ceRNAs) to bind to microRNAs (miRNAs) to regulate gene expression. Previous studies have demonstrated that ceRNAs play an important role in the development of tumors. However, it is not clear whether the lncRNA\u2013miRNA\u2013mRNA ceRNA network plays a role in androgenic alopecia (AGA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The hair follicles of three AGA patients and three healthy individuals were collected for high\u2010throughput whole transcriptome sequencing to screen for differentially expressed lncRNAs. Differentially expressed lncRNA target genes were subjected to databases to predict miRNA\u2013mRNA and lncRNA\u2013miRNA relationship pairs, and a ceRNA network was constructed using Cytoscape software. Relative expression was verified by real\u2010time quantitative reverse transcription\u2013polymerase chain reaction (qRT\u2010PCR).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>84 lncRNAs were significantly differentially expressed between the hair follicles of AGA patients and those of healthy individuals; 30 were upregulated, and 54 were downregulated. The top 10 upregulated lncRNAs were ENST00000501520, ENST00000448179, ENST00000318291, ENST00000568280, ENST00000561121, ENST00000376609, ENST00000602414, ENST00000573866, ENST00000513358, and ENST00000564194. The top 10 downregulated lncRNAs were ENST00000566804, ENST00000561973, ENST00000587680, ENST00000569927, ENST00000340444, ENST00000424345, ENST00000589787, NR_024344, NR_073026, and NR_110001. The qRT\u2010PCR validation results and receiver\u2010operating characteristic curve analysis indicated that one upregulated lncRNA, LOXL1\u2010AS1 (ENST00000564194), had the most significant clinical diagnostic potential. After further analysis, it was concluded that LOXL1\u2010AS1 could be used as a sponge to target hsa\u2010miR\u20105193, thereby regulating TP53 expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The ceRNA network\u2010regulating AGA was constructed through high\u2010throughput sequencing. Our study also identified a key lncRNA that is possibly related to the AGA pathological process.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24791","type":"journal-article","created":{"date-parts":[[2022,12,2]],"date-time":"2022-12-02T10:38:27Z","timestamp":1669977507000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Application of <scp>lncRNA<\/scp>\u2013<scp>miRNA<\/scp>\u2013<scp>mRNA ceRNA<\/scp> network analysis in the treatment of androgenic alopecia"],"prefix":"10.1002","volume":"37","author":[{"given":"Hanxiao","family":"Wei","sequence":"first","affiliation":[{"name":"Department of Plastic Surgery Affiliated Hospital of Xuzhou Medical University  Xuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Yi","sequence":"additional","affiliation":[{"name":"Xuzhou Medical University  Xuzhou 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