{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,18]],"date-time":"2026-05-18T15:01:19Z","timestamp":1779116479167,"version":"3.51.4"},"reference-count":13,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T00:00:00Z","timestamp":1669680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>We described a patient who exhibited a gradual increase in carbohydrate antigen 19\u20109 (CA19\u20109) concentrations for 4\u2009years at three hospitals, with no associated clinical manifestations; however, we were unable to define the cause of this increase, forcing us to consider whether it was a false\u2010positive result.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Given the potential for interference, this study used multiple system detection, gradient dilution, Polyethylene glycol (PEG) precipitation and heterophilic antibody blocking assay to evaluate the reliability of CA19\u20109 concentration increase.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Analysis of the patient sample using multiple systems indicated that CA19\u20109 concentrations showed an obvious increase (154.0, and 889.2\u00a0IU\/ml, respectively) using the Cobas E602 and Advia Centaur XP systems, and were within the reference ranges (&lt;10\u00a0IU\/ml) on other modules. PEG precipitation on the Cobas E602 and Advia Centaur XP systems reduced the CA19\u20109 concentration, as did heterophilic blocking tube (HBT\u20106, HBT\u20101) blockade.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CA19\u20109 was incorrectly identified to increase due to the presence of heterophilic antibodies. We recommend that heterophilic antibodies should be evaluated in cases with elevated CA19\u20109 level but no associated clinical manifestations to prevent false positives.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24792","type":"journal-article","created":{"date-parts":[[2022,11,30]],"date-time":"2022-11-30T07:47:18Z","timestamp":1669794438000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Persistent increase of carbohydrate antigen 19\u20109 with an unknown reason: A seven\u2010year follow\u2010up case"],"prefix":"10.1002","volume":"36","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2498-1272","authenticated-orcid":false,"given":"Wei","family":"Zhao","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory China\u2010Japan Friendship Hospital  Beijing 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