{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,29]],"date-time":"2026-01-29T22:57:49Z","timestamp":1769727469156,"version":"3.49.0"},"reference-count":45,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T00:00:00Z","timestamp":1669680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2022,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The intake of <jats:italic>Gynura segetum<\/jats:italic>, a traditional Chinese medicine, may be induce hepatic sinusoidal obstruction syndrome (HSOS). It has a high mortality rate based on the severity of the disease and the absence of therapeutic effectiveness. Therefore, the current study was designed to investigate the effects of bicyclol on HSOS induced by <jats:italic>Gynura segetum<\/jats:italic> and the potential molecular mechanisms.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>Gynura segetum<\/jats:italic> (30\u2009g\/kg) was administered for 4\u2009weeks in the model group, while the bicyclol pretreatment group received bicyclol (200\u2009mg\/kg) administration. Serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), cholesterol (CHO), triglyceride (TG), and liver histological assays were detected to assess HSOS. The gene expressions of cytochrome P450 (<jats:italic>CYP450<\/jats:italic>) isozymes were quantified by real\u2010time PCR. Moreover, hepatocellular apoptosis was detected using the terminal deoxynucleotidyl transferase dUTP nick\u2010end labeling (TUNEL) assay, then apoptosis and autophagy\u2010related markers were determined using Western blot.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>As a result, bicyclol pretreatment is notably protected against <jats:italic>Gynura segetum<\/jats:italic>\u2010induced HSOS, as observed by reducing serum ALT levels, inhibiting the reduction in CHO and TG levels, and alleviating the histopathological changes. Bicyclol pretreatment inhibited the changes in mRNA levels of <jats:italic>CYP450<\/jats:italic> isozymes (including the increase in <jats:italic>CYP2a5<\/jats:italic> and decrease in <jats:italic>CYP2b10<\/jats:italic>, <jats:italic>2c29<\/jats:italic>, <jats:italic>2c37<\/jats:italic>, <jats:italic>3a11<\/jats:italic>, and <jats:italic>7b1<\/jats:italic>). In addition, the upregulation of Bcl\u20102 and the downregulation of LC3\u2010II\/LC3\u2010I proteins expression in HSOS were inhibited with bicyclol pretreatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Bicyclol exerted a protective effect against HSOS induced by <jats:italic>Gynura segetum<\/jats:italic>, which could be attributed to the regulated expressions of <jats:italic>CYP450<\/jats:italic> isozymes and alleviated the downregulation of autophagy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24793","type":"journal-article","created":{"date-parts":[[2022,11,30]],"date-time":"2022-11-30T07:42:36Z","timestamp":1669794156000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of bicyclol on hepatic sinusoidal obstruction syndrome induced by <i>Gynura segetum<\/i>"],"prefix":"10.1002","volume":"36","author":[{"given":"Jianzuo","family":"Yao","sequence":"first","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery Li Huili Hospital Affiliated to Ningbo University  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jingyi","family":"Wu","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Zhejiang Pharmaceutical University  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu","family":"Jia","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Zhejiang Pharmaceutical University  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8642-4194","authenticated-orcid":false,"given":"Jingping","family":"Shao","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Zhejiang Pharmaceutical University  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xie","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pharmacy The affiliated hospital of Ningbo university, LiHuiLi Hospital  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5703-4194","authenticated-orcid":false,"given":"Zeping","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Pharmacy The affiliated hospital of Ningbo university, LiHuiLi Hospital  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui","family":"Zhang","sequence":"additional","affiliation":[{"name":"The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical University Wenzhou Medical University  Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary and Pancreatic Surgery Li Huili Hospital Affiliated to Ningbo University  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0806-846X","authenticated-orcid":false,"given":"Xiaomin","family":"Yao","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy Zhejiang Pharmaceutical University  Ningbo China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2022,11,29]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.22772"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(12)60004-X"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/1751-2980.12112"},{"issue":"7","key":"e_1_2_10_5_1","first-page":"762","article-title":"pyrrolizidine alkaloids\u2010containing Chinese medicines in the Chinese pharmacopoeia and related safety concerns","volume":"46","author":"Tang J","year":"2011","journal-title":"Yao Xue Xue Bao"},{"key":"e_1_2_10_6_1","first-page":"1627","article-title":"Expert consensus on diagnosis and treatment of pyrrolizidine alkaloids\u2010related sinusoidal obstruction syndrome (2017, Nanjing)","volume":"33","author":"Cooperative Group for Health and Gall Diseases. 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