{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T21:22:47Z","timestamp":1792790567450,"version":"4.5.0"},"reference-count":51,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,26]],"date-time":"2022-12-26T00:00:00Z","timestamp":1672012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Acinetobacter baumannii<\/jats:italic>\n                      is a pathogen responsible for nosocomial infections, especially in patients with burns and ventilator\u2010associated pneumonia (VAP). The aims of this study was to compare the biofilm formation capacity, antimicrobial resistance patterns and molecular typing based on PFGE (Pulsed\u2010Field Gel Electrophoresis) in\n                      <jats:italic>A.\u00a0baumannii<\/jats:italic>\n                      isolated from burn and VAP patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      A total of 50\n                      <jats:italic>A.\u00a0baumannii<\/jats:italic>\n                      isolates were obtained from burn and VAP patients. In this study, we assessed antimicrobial susceptibility, biofilm formation capacity, PFGE fingerprinting, and the distribution of biofilm\u2010related genes (\n                      <jats:italic>csuD, csuE, ptk, ataA,<\/jats:italic>\n                      and\n                      <jats:italic>ompA<\/jats:italic>\n                      ).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Overall, 74% of the strains were multidrug resistant (MDR), and 26% were extensively drug\u2010resistant (XDR). Regarding biofilm formation capacity, 52%, 36%, and 12% of the isolates were strong, moderate, and weak biofilm producers. Strong biofilm formation capacity significantly correlated with XDR phenotype (12\/13, 92.3%). All the isolates harbored at least one biofilm\u2010related gene. The most prevalent gene was\n                      <jats:italic>csuD<\/jats:italic>\n                      (98%), followed by\n                      <jats:italic>ptk<\/jats:italic>\n                      (90%),\n                      <jats:italic>ataA<\/jats:italic>\n                      (88%),\n                      <jats:italic>ompA<\/jats:italic>\n                      (86%), and\n                      <jats:italic>csuE<\/jats:italic>\n                      (86%). Harboring all the biofilm\u2010related genes was significantly associated with XDR phenotype. Finally, PFGE clustering revealed 6 clusters, among which cluster No. 2 showed a significant correlation with strong biofilm formation and XDR phenotype.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Our findings revealed the variable distribution of biofilm\u2010related genes among MDR and XDR\n                      <jats:italic>A.\u00a0baumannii<\/jats:italic>\n                      isolates from burn and VAP patients. A significant correlation was found between strong biofilm formation capacity and XDR phenotype. Finally, our results suggested that XDR phenotype was predominant among strong\u2010biofilm producer\n                      <jats:italic>A.\u00a0baumannii<\/jats:italic>\n                      in our region.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.24814","type":"journal-article","created":{"date-parts":[[2022,12,27]],"date-time":"2022-12-27T00:46:05Z","timestamp":1672101965000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":33,"title":["Antimicrobial resistance and biofilm formation capacity among\n                    <i>Acinetobacter baumannii<\/i>\n                    strains isolated from patients with burns and ventilator\u2010associated pneumonia"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5143-3178","authenticated-orcid":false,"given":"Saeed","family":"Khoshnood","sequence":"first","affiliation":[{"name":"Clinical Microbiology Research Center Ilam University of Medical Sciences  Ilam Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nourkhoda","family":"Sadeghifard","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Research Center Ilam University of Medical Sciences  Ilam Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nahid","family":"Mahdian","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine Ilam University of Medical Sciences  Ilam Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9839-5017","authenticated-orcid":false,"given":"Mohsen","family":"Heidary","sequence":"additional","affiliation":[{"name":"Cellular and Molecular Research Center Sabzevar University of Medical Sciences  Sabzevar Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Somayeh","family":"Mahdian","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Research Center Ilam University of Medical Sciences  Ilam Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryam","family":"Mohammadi","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Faculty of Medicine Ilam University of Medical Sciences  Ilam Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abbas","family":"Maleki","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Research Center Ilam University of Medical Sciences  Ilam Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad Hossein","family":"Haddadi","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Research Center Ilam University of Medical Sciences  Ilam Iran"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2022,12,26]]},"reference":[{"issue":"10","key":"e_1_2_10_2_1","first-page":"1","article-title":"Antibacterial resistance pattern of Acinetobacter baumannii in burn patients in Northeast of Iran. 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