{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T15:01:40Z","timestamp":1777561300806,"version":"3.51.4"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,3,1]],"date-time":"2023-03-01T00:00:00Z","timestamp":1677628800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81673937"],"award-info":[{"award-number":["81673937"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Rheumatoid arthritis (RA) is a persistent and systemic autoimmunity disease. The abnormal differentiation of Treg cells is important in pathogenesis. Despite previous studies showed that microRNAs (miRNAs, miR) are pivotal modulators of Treg cells, the effect of miRNAs on Treg cell differentiation and function is not clear. Our study wants to reveal the relationship of miR\u2010143\u20103p with the differentiative ability and biofunction of Treg cells during the development of RA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The Expressing level of miR\u2010143\u20103p and cell factor generation in peripheral blood (PB) of RA sufferers were identified by ELISA or RT\u2010qPCR. The roles of miR\u2010143\u20103p in Treg cell differentiation were studied via ShRNA\/lentivirus transfection. Male DBA\/1\u00a0J mice were separated into control, model, control mimics, and miR\u2010143\u20103p mimics groups to analyze the anti\u2010arthritis efficacy, the differentiative ability of Treg cells, and the expression level of miR\u2010143\u20103p.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our team discovered that the Expressing level of miR\u2010143\u20103p was related to RA disease activities in a negative manner, and remarkably related to antiinflammation cell factor IL\u201010. In vitro, the expression of miR\u2010143\u20103p in the CD4<jats:sup>+<\/jats:sup>T cells upregulated the percentage of CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>+<\/jats:sup>Fxop3<jats:sup>+<\/jats:sup> cells (Tregs) and forkhead box protein 3 (Foxp3) mRNA expression. Evidently, miR\u2010143\u20103p mimic intervention considerably upregulated the content of Treg cells in vivo, validly avoided CIA progression, and remarkably suppressed the inflammatory events of joints in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings indicated that miR\u2010143\u20103p could ameliorate CIA through polarizing naive CD4<jats:sup>+<\/jats:sup>T cells into Treg cells, which may be a novel strategy to treat autoimmune diseases such as RA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24845","type":"journal-article","created":{"date-parts":[[2023,3,2]],"date-time":"2023-03-02T07:54:37Z","timestamp":1677743677000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["<scp>MicroRNA<\/scp>\u2010143\u20103p ameliorates collagen\u2010induced arthritis by polarizing naive <scp>CD4<\/scp><sup>+<\/sup>T cells into Treg cells"],"prefix":"10.1002","volume":"37","author":[{"given":"Mei\u2010Yu","family":"Shen","sequence":"first","affiliation":[{"name":"School of Pharmacy, Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bao\u2010Ping","family":"Jiang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica Nanjing University of Chinese Medicine  Nanjing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ming\u2010Fei","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Jiangsu Key Laboratory for Pharmacology and Safety Evaluation of Chinese Materia Medica Nanjing University of Chinese Medicine  Nanjing 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