{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T12:19:18Z","timestamp":1790943558078,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T00:00:00Z","timestamp":1678752000000},"content-version":"vor","delay-in-days":13,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2023,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The presence of isolated elevated serum amylase levels can be caused by high molecular mass complexes. We describe 13 cases of hyperamylasemia detected in adult patients without clinical symptoms of a pancreatic disorder. Five of them were thoroughly examined using different tools for the detection of macrocomplexes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We performed both screening and more advanced methods of macroamylase detection, including polyethylene glycol precipitation, sample storage at 4\u00b0C and separation by gel filtration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The presence of macroamylase in the suspected samples was confirmed by the methods described, except for the sample storage at 4\u00b0C. In this method, the enzyme activity did not decrease. The polyethylene glycol precipitation activity (% PPA) averaged 89.1% for amylase, whereas the control samples averaged 30.7%. Gel filtration chromatography confirmed an IgA macroamylase peak in three samples and an IgG macroamylase peak in two samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The presence of macroamylase should be suspected whenever the clinical history and condition of the patient do not match the measured enzyme value to avoid diagnostic errors and unnecessary invasive examinations. The presence of macrocomplexes is considered a benign process that may occur in apparently healthy individuals. Cooperation between clinicians and laboratory staff is necessary.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24859","type":"journal-article","created":{"date-parts":[[2023,3,14]],"date-time":"2023-03-14T12:29:08Z","timestamp":1678796948000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Significant elevations of serum amylase caused by macroamylase: Case reports and detection possibilities"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2499-7194","authenticated-orcid":false,"given":"Jitka","family":"\u010c\u00e1sensk\u00e1","sequence":"first","affiliation":[{"name":"Department of Laboratory Methods Institute for Clinical and Experimental Medicine  Praha Czech Republic"},{"name":"Third Faculty of Medicine Charles University  Praha Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5094-7724","authenticated-orcid":false,"given":"Janka","family":"Franekov\u00e1","sequence":"additional","affiliation":[{"name":"Department of Laboratory Methods Institute for Clinical and Experimental Medicine  Praha Czech Republic"},{"name":"Third Faculty of Medicine Charles University  Praha Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3699-6914","authenticated-orcid":false,"given":"Peter","family":"Ma\u010dinga","sequence":"additional","affiliation":[{"name":"Department of Hepatology and Gastroenterology Institute for Clinical and Experimental Medicine  Praha Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4144-3129","authenticated-orcid":false,"given":"Anton\u00edn","family":"Jabor","sequence":"additional","affiliation":[{"name":"Department of Laboratory Methods Institute for Clinical and Experimental Medicine  Praha Czech Republic"},{"name":"Third Faculty of Medicine Charles University  Praha Czech Republic"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,3,14]]},"reference":[{"key":"e_1_2_9_2_1","volume-title":"Clinical Laboratory Diagnostics: Use and Assessment of Clinical Laboratory Results","author":"Thomas L","year":"1998"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/31.6.1078"},{"key":"e_1_2_9_4_1","first-page":"1326","article-title":"A case of ulcerative colitis with positive alkaline phosphatase\u2010linked immunoglobulin","volume":"86","author":"Horie Y","year":"1989","journal-title":"Nihongo J Sh\u014dkaki"},{"key":"e_1_2_9_5_1","first-page":"41","article-title":"The CK and LD macroenzymes","volume":"29","author":"Pesce MA","year":"1984","journal-title":"Lab Manage"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0025-6196(12)60129-3"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12916-017-0784-x"},{"key":"e_1_2_9_8_1","first-page":"279","article-title":"Macroamylasemia: a simple stepwise approach to diagnosis","volume":"2","author":"LeVine D","year":"1989","journal-title":"J Am Board Fam Pract"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0016-5085(82)80331-4"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1258\/000456303322326425"},{"key":"e_1_2_9_11_1","doi-asserted-by":"publisher","DOI":"10.5152\/tjg.2014.7504"},{"key":"e_1_2_9_12_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJGM.S224281"},{"key":"e_1_2_9_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1754.2004.00494.x"},{"key":"e_1_2_9_14_1","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-60-6-1053"},{"key":"e_1_2_9_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/s0009-9120(86)80036-4"},{"key":"e_1_2_9_16_1","first-page":"54","article-title":"The prevalence of macroamylasemia. 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