{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T00:12:28Z","timestamp":1793059948177,"version":"4.5.0"},"reference-count":14,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,5,9]],"date-time":"2023-05-09T00:00:00Z","timestamp":1683590400000},"content-version":"vor","delay-in-days":38,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81972000"],"award-info":[{"award-number":["81972000"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82172348"],"award-info":[{"award-number":["82172348"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81902139"],"award-info":[{"award-number":["81902139"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate thyroid\u2010stimulating immunoglobulin (TSI) and thyrotropin receptor antibodies (TRAb) diagnostic performance for Graves' disease (GD) and determine clinical cut\u2010off value for diagnosing GD.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Of 1369 retrospectively enrolled subjects, 1364 had a definitive diagnosis of untreated GD (GD\u2010UT, <jats:italic>n<\/jats:italic>\u2009=\u200987); treated GD (GD\u2010T, <jats:italic>n<\/jats:italic>\u2009=\u2009206); autoimmune thyroid disease (AIT, <jats:italic>n<\/jats:italic>\u2009=\u2009241); thyroid nodules (TN, <jats:italic>n<\/jats:italic>\u2009=\u2009677); subacute thyroiditis (ST, <jats:italic>n<\/jats:italic>\u2009=\u200928); healthy subjects (HS, <jats:italic>n<\/jats:italic>\u2009=\u2009125); other diseases with serological hyperthyroidism (<jats:italic>n<\/jats:italic>\u2009=\u20095) and were grouped into the following: UT\u2010GD and control groups (AIT, TN, ST, and HS); and UT\u2010GD and non\u2010GD hyperthyroidism groups. Diagnostic performance of TSI and TRAb was evaluated using area under the curve (AUC) of receiver\u2010operating characteristic (ROC) curve, and optimal clinical cut\u2010off value was determined using maximization of Youden index.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>TRAb AUC and clinical cut\u2010off value for diagnosing GD were 0.981 and 1.245\u2009IU\/L (sensitivity, 96.6%; specificity, 97.1%; positive predictive value [PPV], 71.8%; negative predictive value [NPV], 99.9%; positive likelihood ratio [PLR], 33.31; negative likelihood ratio [NLR, 0.035), respectively, for the GD\u2010UT and control groups. Those for TSI were 0.992 and 0.467\u2009IU\/L (sensitivity 98.8%; specificity, 96.4%; PPV, 68.8%; NPV, 99.9%; PLR, 27.472; NLR, 0.011). Those for TRAb in GD\u2010UT and non\u2010GD hyperthyroidism groups were 0.923 and 1.78\u2009IU\/L (sensitivity, 92.0%; specificity, 89.1%; PPV, 93%; NPV, 87.5%; PLR, 8.44; NLR, 0.089), respectively. For TSI, these were 0.92 and 0.545\u2009IU\/L (sensitivity, 97.7%; specificity, 83.6%; PPV, 90.4%; NPV, 95.8%; PLR27.472, NLR, 0.011), respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TSI diagnostic performance for GD was excellent and had better sensitivity than TRAb.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24890","type":"journal-article","created":{"date-parts":[[2023,5,11]],"date-time":"2023-05-11T01:18:51Z","timestamp":1683767931000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Evaluation of the diagnostic performance of thyroid\u2010stimulating immunoglobulin and thyrotropin receptor antibodies for Graves' disease"],"prefix":"10.1002","volume":"37","author":[{"given":"Shiji","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine Zhongshan Hospital Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqi","family":"Shao","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Zhongshan Hospital Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qun","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Zhongshan Hospital Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Zhongshan Hospital Fudan University  Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baishen","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine Zhongshan Hospital Fudan University  Shanghai 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