{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:46:41Z","timestamp":1786978001753,"version":"build-2736575974"},"reference-count":36,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2023,5,9]],"date-time":"2023-05-09T00:00:00Z","timestamp":1683590400000},"content-version":"vor","delay-in-days":38,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>It is advised to pretreat the reagent erythrocytes with Dithiothreitol (DTT) to denature the surface CD38 to prevent anti\u2010CD38 monoclonal antibodies (MoAb) from interfering with the blood compatibility test. Anti\u2010CD38 has little impact on the Polybrene test, but it is still unknown how sensitive it is to detect irregular antibodies and how effective it is when compared to the standard DTT\u2010based method.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Twenty\u2010one patients receiving daratumumab (\n                      <jats:italic>N<\/jats:italic>\n                      \u2009=\u200913) and isatuximab (\n                      <jats:italic>N<\/jats:italic>\n                      \u2009=\u20098) had their serum collected. Standard anti\u2010sera (anti\u2010c, D, E, Fy\n                      <jats:sup>b<\/jats:sup>\n                      , Jk\n                      <jats:sup>a<\/jats:sup>\n                      , M, Mi\n                      <jats:sup>a<\/jats:sup>\n                      ) with serial dilution were added to patients' serum. Antibody screening tests were performed simultaneously using the manual polybrene method (MP) and DTT\u2010pretreated, automatic indirect antiglobulin test (DTT\u2010IAT) to compare the detection sensitivity. These two methods' operating times and costs were also analyzed.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Both MP and DTT\u2010IAT can overcome the interference caused by anti\u2010CD38 MoAb. However, MP is more sensitive in detecting anti\u2010M and anti\u2010Mi\n                      <jats:sup>a<\/jats:sup>\n                      and is comparable to DTT\u2010IAT in detecting other antibodies. In terms of cost and operating time, MP is also far superior to DTT\u2010IAT.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      MP is a cost\u2010effective alternative to DTT\u2010IAT in resolving anti\u2010CD38 interference and is especially suitable for populations with a high prevalence of anti\u2010M and anti\u2010Mi\n                      <jats:sup>a<\/jats:sup>\n                      . However, both methods have a well\u2010known drawback of low detection sensitivity for anti\u2010K, and K\u2010units should be provided to patients to prevent hemolytic transfusion reactions.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.24891","type":"journal-article","created":{"date-parts":[[2023,5,10]],"date-time":"2023-05-10T21:25:01Z","timestamp":1683753901000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Better resolving of\n                    <scp>anti\u2010CD38<\/scp>\n                    antibody interference with blood compatibility testing by using manual polybrene method\u00a0compared with dithiothreitol\u2010pretreatment indirect antiglobulin test"],"prefix":"10.1002","volume":"37","author":[{"given":"Chiu\u2010Che","family":"Feng","sequence":"first","affiliation":[{"name":"Division of Hematology and Oncology, Department of Internal Medicine China Medical University Hospital  Taichung Taiwan"},{"name":"Graduate Institute of Medicine Kaohsiung Medical University  Kaohsiung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ci\u2010Wen","family":"Chang","sequence":"additional","affiliation":[{"name":"Blood Bank, Department of Laboratory Medicine China Medical University Hospital  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Yi","family":"Lien","sequence":"additional","affiliation":[{"name":"Blood Bank, Department of Laboratory Medicine China Medical University Hospital  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinun\u2010An","family":"Lin","sequence":"additional","affiliation":[{"name":"Blood Bank, Department of Laboratory Medicine China Medical University Hospital  Taichung Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tzu\u2010Ting","family":"Chen","sequence":"additional","affiliation":[{"name":"Division of 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