{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,22]],"date-time":"2026-10-22T15:16:56Z","timestamp":1792682216484,"version":"4.5.0"},"reference-count":30,"publisher":"Wiley","issue":"13-14","license":[{"start":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T00:00:00Z","timestamp":1690416000000},"content-version":"vor","delay-in-days":26,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Acute febrile illness (AFI) is a prevalent disease in developing countries that is difficult to diagnose due to the diversity of infectious organisms and the poor quality of clinical diagnosis. TaqMan array card (TAC) can detect up to 35 AFI\u2010associated organisms in 1.5\u2009h, addressing diagnostic demands. In this study, we aimed to evaluate the role of TAC in determining the causative organisms in hospitalized AFI patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study had a cross\u2010sectional design and enrolled 120 admitted patients with persistent fever for three or more days from the medicine ward of Chittagong Medical College Hospital (CMCH) and Bangladesh Institute of Tropical and Infectious Diseases Hospital (BITID). Blood samples were collected and then subjected to automated BacT\/Alert blood culture, microbial culture, TAC assay, and typhoid\/paratyphoid test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The total number of study participants was 120, among them 48 (40%) samples showed a positive result in TAC card, 29 (24.16%) were TP positive and nine (7.51%) were culture positive. The number of organisms detected by the TAC card was 13 bacteria, three viruses, one protozoan, and one fungus. The sensitivity and specificity of the TAC assay for different bacterial pathogen compared to blood culture was 44.44%, and 90.99%, respectively. In contrast, the TP test had a sensitivity and specificity of 100% and 80%, respectively, compared to the blood culture test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TAC can be a handful tool for detecting multiple organisms in AFI with high specificity which can facilitate early diagnosis of different pathogens contributing to AFI.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24948","type":"journal-article","created":{"date-parts":[[2023,7,27]],"date-time":"2023-07-27T07:17:18Z","timestamp":1690442238000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Role of TaqMan array card in determining causative organisms of acute febrile illness in hospitalized patients"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0991-6598","authenticated-orcid":false,"given":"Tabassum","family":"Ferdousi","sequence":"first","affiliation":[{"name":"Bangladesh Institute of Tropical and Infectious Diseases  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asok Kumar","family":"Dutta","sequence":"additional","affiliation":[{"name":"Chattogram Medical College Hospital  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. A. Hassan","family":"Chowdhury","sequence":"additional","affiliation":[{"name":"Chattogram Medical College Hospital  Chattogram Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamrul","family":"Islam","sequence":"additional","affiliation":[{"name":"International Centre for Diarrheal Disease Research, Bangladesh (icddr,b)  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Taufiqul","family":"Islam","sequence":"additional","affiliation":[{"name":"International Centre for Diarrheal Disease Research, Bangladesh (icddr,b)  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Md. Zahirul","family":"Islam","sequence":"additional","affiliation":[{"name":"Institute for Developing Science and Health Initiatives (ideSHi)  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6655-3087","authenticated-orcid":false,"given":"Md. Rakibul Hassan","family":"Bulbul","sequence":"additional","affiliation":[{"name":"Institute for Developing Science and Health Initiatives (ideSHi)  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ashraful Islam","family":"Khan","sequence":"additional","affiliation":[{"name":"International Centre for Diarrheal Disease Research, Bangladesh (icddr,b)  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Firdausi","family":"Qadri","sequence":"additional","affiliation":[{"name":"International Centre for Diarrheal Disease Research, Bangladesh (icddr,b)  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,7,27]]},"reference":[{"key":"e_1_2_8_2_1","volume-title":"Foundation for innovative new diagnostics (FIND), acute febrile syndrome strategy","year":"2012"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002331"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2012.11-0409"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.20546\/ijcmas.2016.507.076"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-017-2239-6"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-11-323"},{"key":"e_1_2_8_8_1","first-page":"34","article-title":"Sero\u2010diagnosis of dengue infections by haemagglutination inhibition test (HI) in suspected cases in Chattogram, Bangladesh","volume":"23","author":"Amin MMM","year":"1999","journal-title":"WHO Dengue Bull"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.13-0570"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1201-9712(01)90027-X"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/jpids\/piw016"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1517\/17530059.2012.667799"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.2016.1112274"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.2015.1029459"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00134-013-3077-7"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa010307"},{"key":"e_1_2_8_17_1","first-page":"65","article-title":"Laboratory diagnosis of invasive candidiasis","volume":"43","author":"Ellepola AN","year":"2005","journal-title":"J Microbiol"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-017-17765-5"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jviromet.2015.11.020"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2016.11.269"},{"key":"e_1_2_8_21_1","volume-title":"C.L.S.I. performance standards for antimicrobial susceptibility testing: twenty\u2010second informational supplement ed. 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