{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T02:26:12Z","timestamp":1784168772649,"version":"3.55.0"},"reference-count":25,"publisher":"Wiley","issue":"17-18","license":[{"start":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T00:00:00Z","timestamp":1694044800000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2023,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The Friedewald or Martin\/Hopkins equation is widely used to estimate low\u2010density lipoprotein cholesterol (LDL\u2010C) at triglyceride (TG) levels &lt;400\u2009mg\/dL. In this study, we aimed to validate the recently developed Sampson and extended Martin\/Hopkins equations intended for use in patients with TG levels up to 800\u2009mg\/dL by comparing them to a direct homogenous assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In total, 8676 participants with serum TG levels &lt;800\u2009mg\/dL were enrolled in this study. LDL\u2010C was directly measured using Abbott homogeneous assay (DLDL) and estimated using the Friedewald (FLDL), Martin\/Hopkins (MLDL), extended Martin\/Hopkins (EMLDL), and Sampson equations (SLDL). The overall concordance between the DLDL and LDL\u2010C estimates was calculated. The performance of the four equations was also compared using Bland\u2013Altman plots and mean absolute difference (MAD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The EMLDL was more accurate than other LDL\u2010C equations particularly for patients with TG\u2265400\u2009mg\/dL (MAD\u2009=\u200910.43; vs. FLDL: MAD\u2009=\u200921.1; vs. SLDL: MAD 11.62). The overall concordance of FLDL, MLDL, EMLDL, and SLDL with DLDL in TG values ranging from 200 to 799\u2009mg\/dL were 52.2, 70.5, 71.6, and 65.7%, respectively (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.001), demonstrating the EMLDL as the most optimal estimation method, particularly for high TG levels (\u2265200\u2009mg\/dL).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Both the original and extended Martin\/Hopkins method are optimal in estimating LDL\u2010C levels in clinical laboratories using the Abbott analyzer in patients with TG levels of 200\u2013399 and 400\u2013799\u2009mg\/dL, respectively. Meanwhile, caution is need that considerable underestimation of Friedewald and Sampson equation could lead to undertreatment in hypertriglyceridemia.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.24963","type":"journal-article","created":{"date-parts":[[2023,9,8]],"date-time":"2023-09-08T05:24:52Z","timestamp":1694150692000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Correlation of extended Martin\/Hopkins equation with a direct homogeneous assay in assessing low\u2010density lipoprotein cholesterol in patients with hypertriglyceridemia"],"prefix":"10.1002","volume":"37","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4528-1534","authenticated-orcid":false,"given":"Soie","family":"Chung","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Healthcare Research Institute, Healthcare System Gangnam Center Seoul National University Hospital  Seoul Republic of Korea"},{"name":"Department of Laboratory Medicine Seoul National University College of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2023,9,7]]},"reference":[{"key":"e_1_2_8_2_1","unstructured":"Who.int [homepage on the internet].World Health Organization: Cardiovascular diseases: fact sheet. [Accessed 2023 April 15]. Available from:https:\/\/www.who.int\/news\u2010room\/fact\u2010sheets\/detail\/cardiovascular\u2010diseases\u2010(cvds)"},{"issue":"25","key":"e_1_2_8_3_1","first-page":"e1082","article-title":"2018 AHA\/ACC\/AACVPR\/AAPA\/ABC\/ACPM\/ADA\/AGS\/APhA\/ASPC\/NLA\/PCNA guideline on the Management of Blood Cholesterol: a report of the American College of Cardiology\/American Heart Association Task Force on Clinical Practice Guidelines","volume":"139","author":"Grundy SM","year":"2019","journal-title":"Circulation"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2013.280532"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.5551\/jat.44396"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.117.030677"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2014.227710"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamacardio.2018.1533"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0148147"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000485977"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.5551\/jat.35972"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamacardio.2020.0013"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-021-92625-x"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1001\/jamanetworkopen.2021.28817"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1515\/cclm-2021-0747"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/18.6.499"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0735-1097(90)90282-T"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijcard.2021.02.009"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2013.01.079"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2018.11.003"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2022.07.006"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/clinchem\/hvad012"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2009.142810"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.15263\/jlmqa.2019.41.3.121"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.12997\/jla.2019.8.2.78"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehz455"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.24963","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,3]],"date-time":"2023-11-03T11:19:50Z","timestamp":1699010390000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.24963"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,9]]},"references-count":25,"journal-issue":{"issue":"17-18","published-print":{"date-parts":[[2023,9]]}},"alternative-id":["10.1002\/jcla.24963"],"URL":"https:\/\/doi.org\/10.1002\/jcla.24963","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,9]]},"assertion":[{"value":"2023-04-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-08-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2023-09-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e24963"}}