{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T00:35:12Z","timestamp":1784334912737,"version":"3.55.0"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2024,1,30]],"date-time":"2024-01-30T00:00:00Z","timestamp":1706572800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2024,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Point\u2010of\u2010care tests (POCTs) may have a role in detecting undiagnosed cases of Celiac disease (CD). We assessed the diagnostic accuracy of a novel POCT, compared with the conventional serological methods, for simultaneous anti\u2010transglutaminase (tTG) IgA and anti\u2010deamidated gliadin (DGP) IgG antibody detection. Furthermore, we evaluated the effect of different biological matrices (whole blood and serum) on test performance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Serum and whole blood from celiac or suspected celiac patients who underwent duodenal biopsy were assayed for the presence of anti\u2010tTG IgA and anti\u2010DGP IgG both with the reference standard methods (Thermo Fisher Scientific, Uppsala, Sweden) and with the POCT (PRIMA Lab SA, Balerna, Switzerland).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>266 sera (101 negative and 165 positive) and 60 whole blood samples (34 positive and 26 negative) were included in the study. POCT for anti\u2010DGP IgG showed a sensitivity of 84.3% and a specificity of 90.1%, with positive (PPV) and negative predictive values (NPV) of 91.07% and 82.73%. POCT for anti\u2010tTG IgA showed a sensitivity of 98.31% and a specificity of 98.02%, with a PPV and NPV of 98.31% and 98.02%. Test accuracies were 86.94% and 98.17%, respectively. The agreement of the results between the two different matrices showed a strong correlation rate: 95% for anti\u2010DGP IgG and 100% for anti\u2010tTG IgA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The anti\u2010tTG IgA\/anti\u2010DGP IgG\u2010based POCT showed good diagnostic accuracy with comparable sensitivities and specificities to reference standard methods in detecting CD in symptomatic patients and could be considered as a mass screening test before referring to conventional serology.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.25003","type":"journal-article","created":{"date-parts":[[2024,1,31]],"date-time":"2024-01-31T06:53:16Z","timestamp":1706683996000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Diagnostic accuracy of a novel point\u2010of\u2010care test for simultaneous detection of anti\u2010transglutaminase <scp>IgA<\/scp> and anti\u2010deamidated gliadin <scp>IgG<\/scp> antibodies"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1510-2718","authenticated-orcid":false,"given":"Giuseppe","family":"Parrinello","sequence":"first","affiliation":[{"name":"Immunology and Allergy Unit Santa Maria degli Angeli Hospital  Pordenone Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9852-1431","authenticated-orcid":false,"given":"Mirella","family":"Da\u00a0Re","sequence":"additional","affiliation":[{"name":"Immunology and Allergy Unit Santa Maria degli Angeli Hospital  Pordenone Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Grizzo","sequence":"additional","affiliation":[{"name":"Immunology and Allergy Unit Santa Maria degli Angeli Hospital  Pordenone 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