{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,27]],"date-time":"2026-06-27T12:32:39Z","timestamp":1782563559343,"version":"3.54.5"},"reference-count":42,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T00:00:00Z","timestamp":1716336000000},"content-version":"vor","delay-in-days":21,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100020824","name":"Invasive Fungi Research Center, Mazandaran University of Medical Sciences","doi-asserted-by":"publisher","award":["10368"],"award-info":[{"award-number":["10368"]}],"id":[{"id":"10.13039\/501100020824","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The current study aimed to identify Iranian <jats:italic>Nakaseomyces<\/jats:italic> (<jats:italic>Candida<\/jats:italic>) <jats:italic>glabrata<\/jats:italic> complex species in the clinical isolates and determine their antifungal susceptibility profile.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In total, 320 <jats:italic>N.\u2009glabrata<\/jats:italic> clinical isolates were collected from patients hospitalized in different geographical regions of Iran. The initial screening was performed by morphological characteristics on CHROMagar <jats:italic>Candida<\/jats:italic>. Each isolate was identified by targeting the D1\/D2 rDNA using a multiplex\u2010PCR method. To validate the mPCR method and determine genetic diversity, the ITS\u2010rDNA region was randomly sequenced in 40 isolates. Additionally, antifungal susceptibility was evaluated against nine antifungal agents following the CLSI M27\u2010A4 guidelines.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All clinical isolates from Iran were identified as <jats:italic>N.\u2009glabrata<\/jats:italic>. The analysis of ITS\u2010rDNA sequence data revealed the presence of eight distinct ITS clades and 10 haplotypes among the 40 isolates of <jats:italic>N.\u2009glabrata<\/jats:italic>. The predominant clades identified were Clades VII, V, and IV, which respectively accounted for 22.5%, 17.5%, and 17.5% isolates. The widest MIC ranges were observed for voriconazole (0.016\u20138\u2009\u03bcg\/mL) and isavuconazole (0.016\u20132\u2009\u03bcg\/mL), whereas the narrowest ranges were seen with itraconazole and amphotericin B (0.25\u20132\u2009\u03bcg\/mL).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Haplotype diversity can be a valuable approach for studying the genetic diversity, transmission patterns, and epidemiology of the <jats:italic>N.\u2009glabrata<\/jats:italic> complex.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.25042","type":"journal-article","created":{"date-parts":[[2024,5,22]],"date-time":"2024-05-22T09:28:16Z","timestamp":1716370096000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Molecular Epidemiology and Antifungal Susceptibility Profile in <i>Nakaseomyces glabrata<\/i> Species Complex: A 5\u2010Year Countrywide 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