{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:36:54Z","timestamp":1791243414203,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T00:00:00Z","timestamp":1716854400000},"content-version":"vor","delay-in-days":27,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003710","name":"Korea Health Industry Development Institute","doi-asserted-by":"publisher","award":["RS\u20102024\u201000332244"],"award-info":[{"award-number":["RS\u20102024\u201000332244"]}],"id":[{"id":"10.13039\/501100003710","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Rapid antimicrobial susceptibility testing (AST) for bloodstream infections (BSIs) facilitates the optimization of antimicrobial therapy, preventing antimicrobial resistance and improving patient outcomes. QMAC\u2010dRAST (QuantaMatrix Inc., Korea) is a rapid AST platform based on microfluidic chip technology that performs AST directly using positive blood culture broth (PBCB). This study evaluated the performance of QMAC\u2010dRAST for Gram\u2010negative bacteria using PBCB and subcultured colony isolates, comparing it with that of VITEK 2 (bioM\u00e9rieux, France) using broth microdilution (BMD) as the reference method.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We included 141 Gram\u2010negative blood culture isolates from patients with BSI and 12 carbapenemase\u2010producing clinical isolates of Enterobacterales spiked into blood culture bottles. QMAC\u2010dRAST performance was evaluated using PBCB and colony isolates, whereas VITEK 2 and BMD were tested only on colony isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>For PBCB, QMAC\u2010dRAST achieved 92.1% categorical agreement (CA), 95.3% essential agreement (EA), with 1.8% very major errors (VMEs), 3.5% major errors (MEs), and 5.2% minor errors (mEs). With colony isolates, it exhibited 92.5% CA and 95.1% EA, with 2.0% VMEs, 3.2% MEs, and 4.8% mEs. VITEK 2 showed 94.1% CA and 96.0% EA, with 4.3% VMEs, 0.4% MEs, and 4.3% mEs. QMAC\u2010dRAST yielded elevated error rates for specific antimicrobial agents, with high VMEs for carbapenems and aminoglycosides. The median time to result for QMAC\u2010dRAST was 5.9\u2009h for PBCB samples and 6.1\u2009h for subcultured colony isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The QMAC\u2010dRAST system demonstrated considerable strengths and comparable performance to the VITEK 2 system; however, challenges were discerned with specific antimicrobial agents, underlining a necessity for improvement.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.25043","type":"journal-article","created":{"date-parts":[[2024,5,28]],"date-time":"2024-05-28T12:20:31Z","timestamp":1716898831000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Evaluation of the <scp>QMAC\u2010<\/scp>d<scp>RAST<\/scp> System Version 2.5 for Rapid Antimicrobial Susceptibility Testing of Gram\u2010Negative Bacteria From Positive Blood Culture Broth and Subcultured Colony Isolates"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6405-5305","authenticated-orcid":false,"given":"Tae\u00a0Yeul","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine and Genetics, Samsung Medical Center Sungkyunkwan University School of Medicine  Seoul Republic of Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0330-7828","authenticated-orcid":false,"given":"Minhee","family":"Kang","sequence":"additional","affiliation":[{"name":"Biomedical Engineering Research Center, Smart Healthcare Research Institute Samsung Medical Center  Seoul Republic of Korea"},{"name":"Department of Medical Device Management and Research, Samsung Advanced Institute for Health Science &amp; Technology Sungkyunkwan University  Seoul Republic of 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