{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T02:24:21Z","timestamp":1790216661387,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2024,6,9]],"date-time":"2024-06-09T00:00:00Z","timestamp":1717891200000},"content-version":"vor","delay-in-days":39,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LQ19H030003"],"award-info":[{"award-number":["LQ19H030003"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100008856","name":"Department of Health of Zhejiang Province","doi-asserted-by":"publisher","award":["2019RC302"],"award-info":[{"award-number":["2019RC302"]}],"id":[{"id":"10.13039\/501100008856","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2024,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Severe acute pancreatitis (SAP) is associated with tremendous systemic inflammation, T\u2010helper 17 (Th17) cells, and regulatory T (Treg) cells play an essential role in the inflammatory responses. Meanwhile, soluble fibrinogen\u2013like protein 2 (Sfgl2) is a critical immunosuppressive effector cytokine of Treg cells and modulates immune responses. However, the impact of SAP induction on Sfgl2 expression and the role of Sfgl2 in immunomodulation under SAP conditions are largely unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A taurocholate\u2010induced mouse SAP model was established. The ratios of CD4<jats:sup>+<\/jats:sup>CD25<jats:sup>+<\/jats:sup>Foxp3<jats:sup>+<\/jats:sup> Treg cells or CD4<jats:sup>+<\/jats:sup>IL\u201017<jats:sup>+<\/jats:sup> Th17 cells in blood and pancreatic tissues as well as surface expression of CD80, CD86, and major histocompatibility complex class II (MHC\u2010II) were determined by flow cytometry. Gene mRNA expression was determined by qPCR. Serum amylase and soluble factors were quantitated by commercial kits. Bone marrow\u2013derived dendritic cells (DCs) were generated, and NF\u2010\u03baB\/p65 translocation was measured by immunofluorescence staining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>SAP induction in mice decreased the Th17\/Treg ratio in the pancreatic tissue and increased the Th17\/Treg ratio in the peripheral blood. In addition, SAP was associated with a reduced level of Sfgl2 in the pancreatic tissue and blood: higher levels of serum IL\u201017, IL\u20102, IFN\u2010\u03b1, and TNF\u2010\u03b1, and lower levels of serum IL\u20104 and IL\u201010. Furthermore, the SAP\u2010induced reduction in Sfgl2 expression was accompanied by dysregulated maturation of bone marrow\u2013derived DCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>SAP causes reduced Sfgl2 expression and Th17\/Treg imbalance, thus providing critical insights for the development of Sfgl2\u2010 and Th17\/Treg balance\u2010targeted immunotherapies for patients with SAP.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.25076","type":"journal-article","created":{"date-parts":[[2024,6,10]],"date-time":"2024-06-10T04:55:09Z","timestamp":1717995309000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Soluble Fibrinogen\u2013Like Protein 2 Downregulation and Th17\/Treg Imbalance in a Taurocholate\u2010Induced Murine Experimental Model of Severe Acute Pancreatitis"],"prefix":"10.1002","volume":"38","author":[{"given":"Yibing","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Gastroenterology, Affiliated Jinhua Hospital Zhejiang University School of Medicine  Jinhua Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Affiliated Jinhua Hospital Zhejiang University School of Medicine  Jinhua Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanping","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Affiliated Jinhua Hospital Zhejiang University School of Medicine  Jinhua Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qunying","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Affiliated Jinhua Hospital Zhejiang University School of Medicine  Jinhua Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyun","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Affiliated Jinhua Hospital Zhejiang University School of Medicine  Jinhua Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongjun","family":"Hua","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Affiliated Jinhua Hospital Zhejiang University School of Medicine  Jinhua Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-8590-9880","authenticated-orcid":false,"given":"Xiaohua","family":"Ye","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, Affiliated Jinhua Hospital Zhejiang University School of Medicine  Jinhua Zhejiang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,6,9]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12876-023-02801-8"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(14)60649-8"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i38.13879"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/bjs.7304"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.dld.2011.02.020"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-015-1192-3"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2018.12.041"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.15.1427"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000499138"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00285.2020"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/MCG.0b013e31828a6cfc"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v29.i18.2747"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.4240\/wjgs.v15.i3.307"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i45.16935"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2019.11.002"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd3794"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms20143394"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2016.07.006"},{"key":"e_1_2_12_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0000000000021491"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2023.109799"},{"key":"e_1_2_12_22_1","doi-asserted-by":"crossref","DOI":"10.1002\/brb3.1261","article-title":"Serum Soluble Fibrinogen\u2010Like Protein 2 Concentration Predicts Delirium After Acute Pancreatitis","volume":"9","author":"Xu W. 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