{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:29:10Z","timestamp":1790710150529,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"19-20","license":[{"start":{"date-parts":[[2024,9,25]],"date-time":"2024-09-25T00:00:00Z","timestamp":1727222400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2024,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Interstitial lung diseases (ILD) is a group of lung disorders characterized by interstitial lung thickening due to inflammatory and fibrotic processes. Krebs von den Lungen\u20106 (KL\u20106) is a molecule secreted by damaged type II alveolar pneumocytes in the alveolar space. The goal of the present study was to compare two detection methods of KL\u20106 in both bronchoalveolar lavage (BAL) and serum from ILD patients at the moment of diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Patients with suspicious of ILD and followed at two Italian referral centres for rare lung diseases were included in the study. BAL fluid and serum were collected and analysed by chemiluminescent enzyme immunoassay (CLEIA) and fluorescent enzyme immunoassay (FEIA) methods provided by Tosoh Biosciences.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 158 (mean age\u2009\u00b1\u2009standard deviation, 61.5\u2009\u00b1\u200913.7, 65 females) patients were enrolled. A total of, 36 had diagnosis of idiopathic pulmonary fibrosis (IPF), 74 sarcoidosis, 15 connective tissue disease\u2010ILD (CTD\u2010ILD) and 33 other ILD. Diagnostic agreement between two methods was demonstrated for both BAL (<jats:italic>r<\/jats:italic>\u2009=\u20090.707, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001) and serum (<jats:italic>r<\/jats:italic>\u2009=\u20090.816, <jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). BAL KL\u20106 values were lower than serum (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.0001). IPF patients had higher serum KL\u20106 concentration than other ILDs (<jats:italic>p<\/jats:italic>\u2009=\u20090.0294), while BAL KL\u20106 values were lower in IPF than in non\u2010IPF (<jats:italic>p<\/jats:italic>\u2009=\u20090.0023).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study explored KL\u20106 concentrations through the CLEIA method in serum and BAL of patients with various ILDs, showing significant differences of biomarkers concentrations between IPF and other non\u2010IPF ILDs. Our findings are promising as they provided further knowledge concerning KL\u20106 expression across different ILDs and may suggest its utility in differential diagnosis.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.25108","type":"journal-article","created":{"date-parts":[[2024,9,26]],"date-time":"2024-09-26T06:54:18Z","timestamp":1727333658000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Diagnostic Performance of CLEIA Versus FEIA for KL\u20106 Peripheral and Alveolar Concentrations in Fibrotic Interstitial Lung Diseases: A Multicentre Study"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2368-5722","authenticated-orcid":false,"given":"Miriana","family":"d'Alessandro","sequence":"first","affiliation":[{"name":"Respiratory Diseases Unit, Department of Medical and Surgical Sciences &amp; Neuro\u2010Sciences University of Siena  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sara","family":"Gangi","sequence":"additional","affiliation":[{"name":"Respiratory Diseases Unit, Department of Medical and Surgical Sciences &amp; Neuro\u2010Sciences University of Siena  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Paggi","sequence":"additional","affiliation":[{"name":"Respiratory Diseases Unit, Department of Medical and Surgical Sciences &amp; Neuro\u2010Sciences University of Siena  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piera","family":"Soccio","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences University of Foggia  Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Bergantini","sequence":"additional","affiliation":[{"name":"Respiratory Diseases Unit, Department of Medical and Surgical Sciences &amp; Neuro\u2010Sciences University of Siena  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tommaso","family":"Pianigiani","sequence":"additional","affiliation":[{"name":"Respiratory Diseases Unit, Department of Medical and Surgical Sciences &amp; Neuro\u2010Sciences University of Siena  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giusy","family":"Montuori","sequence":"additional","affiliation":[{"name":"Respiratory Diseases Unit, Department of Medical and Surgical Sciences &amp; Neuro\u2010Sciences University of Siena  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giorgia","family":"Moriondo","sequence":"additional","affiliation":[{"name":"Department of Medical and Surgical Sciences University of Foggia  Foggia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giulio","family":"Natalello","sequence":"additional","affiliation":[{"name":"Respiratory Diseases Unit, Department of Medical and Surgical Sciences &amp; Neuro\u2010Sciences University of Siena  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sofia","family":"Marrucci","sequence":"additional","affiliation":[{"name":"Respiratory Diseases Unit, Department of Medical and Surgical Sciences &amp; 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