{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:46:41Z","timestamp":1786978001549,"version":"build-2736575974"},"reference-count":50,"publisher":"Wiley","issue":"22","license":[{"start":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T00:00:00Z","timestamp":1728518400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2024,11]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Tuberculosis (TB) is a preventable and treatable disease leading to the second death globally. The evolution of drug resistance in\n                      <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                      (MTB), particularly rifampicin resistance (RR), has hampered TB control efforts. Thus, this study aimed to provide information regarding the magnitude of MTB and rifampicin resistance among patients tested using the GeneXpert method.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      A retrospective analysis was carried out at DTCSH. The study included TB registration logbook data from all patients who visited the hospital and were tested for MTB with the Xpert MTB\/RIF assay from 2017 to 2024. The laboratory\u2010based data were entered, cleaned, and analyzed using SPSS version 26 software. Multilogistic regression analysis was employed, and a\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0value \u2264\u20090.05 was considered statistically significant.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 12,981 patient results were included, of which 8.9% (1160\/12,981) were MTB\u2010positive and 7.1% (82\/1160) were RR. Individuals aged 15\u201329\u2009years (AOR\u2009=\u20092.13; 95% CI\u2009=\u20091.55\u20132.93,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001), living in rural areas (AOR\u2009=\u20091.23; 95% CI\u2009=\u20091.08\u20131.41,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009=\u20090.003), and HIV+ (AOR\u2009=\u20091.79; 95% CI\u2009=\u20091.48\u20132.33,\n                      <jats:italic>p<\/jats:italic>\n                      \u2009&lt;\u20090.001) had a higher risk of developing tuberculosis. While RR was identified in 63.4% (52\/82) of new, 24.4% (20\/82) of re\u2010treated, and 12.2% (10\/82) of failed presumptive TB patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>In this study, MTB and RR trends were high. Productive age groups, rural populations, and HIV patients were at risk. To lessen the burden of this contagious and fatal disease, it is recommended to increase early diagnosis of drug\u2010resistant TB and enhance infection control.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/jcla.25111","type":"journal-article","created":{"date-parts":[[2024,10,10]],"date-time":"2024-10-10T10:50:27Z","timestamp":1728557427000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Detection of Rifampicin Resistance\n                    <scp>rpoB<\/scp>\n                    Gene Using\n                    <scp>GeneXpert MTB<\/scp>\n                    \/\n                    <scp>RIF<\/scp>\n                    Assay in Pulmonary Tuberculosis Cases at Debre Tabor Comprehensive Specialized Hospital, Northwest Ethiopia"],"prefix":"10.1002","volume":"38","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7575-2387","authenticated-orcid":false,"given":"Bekele","family":"Sharew","sequence":"first","affiliation":[{"name":"Department of Medical Laboratory Science College of Health Sciences, Debre Tabor University  Debre Tabor Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayenew","family":"Berhan","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science College of Health Sciences, Debre Tabor University  Debre Tabor Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andargachew","family":"Almaw","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science College of Health Sciences, Debre Tabor University  Debre Tabor Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mulat","family":"Erkihun","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science College of Health Sciences, Debre Tabor University  Debre Tabor Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tegenaw","family":"Tiruneh","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science College of Health Sciences, Debre Tabor University  Debre Tabor Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Teklehaimanot","family":"Kiros","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science College of Health Sciences, Debre Tabor University  Debre Tabor Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2033-5663","authenticated-orcid":false,"given":"Yenealem","family":"Solomon","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science College of Health Sciences, Debre Tabor University  Debre Tabor Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitikie","family":"Wondmagegn","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science College of Health Sciences, Debre Tabor University  Debre Tabor Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Etenesh","family":"Wondimu","sequence":"additional","affiliation":[{"name":"College of Veterinary Medicine, Bonga University  Bonga Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abay","family":"Teshager","sequence":"additional","affiliation":[{"name":"Debre Tabor Comprehensive Specialized Hospital  Debre Tabor Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simegnew","family":"Bihonegn","sequence":"additional","affiliation":[{"name":"Debre Tabor Comprehensive Specialized Hospital  Debre Tabor Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihret","family":"Tilahun","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science College of Medicine and Health Sciences, Wollo University  Wollo Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birhanu","family":"Getie","sequence":"additional","affiliation":[{"name":"Department of Medical Laboratory Science College of Health Sciences, Debre Tabor University  Debre Tabor Ethiopia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,10,10]]},"reference":[{"key":"e_1_2_11_2_1","volume-title":"Sustainable Development Goals","year":"2022"},{"key":"e_1_2_11_3_1","volume-title":"Coronavirus (COVID\u201019) Dashboard","year":"2022"},{"key":"e_1_2_11_4_1","volume-title":"Global Tuberculosis Report 2023","year":"2023"},{"key":"e_1_2_11_5_1","volume-title":"Global Strategy and Targets for Tuberculosis Prevention, Care and Control After 2015","year":"2014"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.5588\/ijtld.17.0835"},{"key":"e_1_2_11_7_1","volume-title":"Global Tuberculosis Report 2022","year":"2022"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.5588\/ijtld.13.0417"},{"key":"e_1_2_11_9_1","volume-title":"WHO Global Lists of High Burden Countries for Tuberculosis (TB), TB\/HIV and Multidrug\/Rifampicin\u2010Resistant TB (MDR\/RR\u2010TB), 2021\u20132025: Background Document?","author":"World Health Organization","year":"2021"},{"key":"e_1_2_11_10_1","volume-title":"Global Tuberculosis Report 2021","year":"2021"},{"key":"e_1_2_11_11_1","unstructured":"Federal Ministry of Health (FMoH) Ethiopia \u201cGuidelines for Clinical and Programmatic Management of TB TB\/HIV DR\u2010TB and Leprosy in Ethiopia \u201d (2021)."},{"key":"e_1_2_11_12_1","unstructured":"Federal Democratic Republic of Ethiopia FMoH\/EPHI \u201cImplementation Guideline for GeneXpert MTB\/RIF Assay in Ethiopia \u201d (2014)."},{"issue":"11","key":"e_1_2_11_13_1","doi-asserted-by":"crossref","DOI":"10.1371\/journal.pone.0225205","article-title":"Monitoring Quality Indicators for the Xpert MTB\/RIF Molecular Assay in Ethiopia","volume":"14","author":"Alemu A.","year":"2019","journal-title":"PLoS One"},{"key":"e_1_2_11_14_1","unstructured":"Federal Democratic Republic of Ethiopia Ministry of Health (FDRE FMoH)\/Ethiopian Public Health Institute (EPHI) \u201cImplementation Guideline for GeneXpert MTB\/RIF Assay in Ethiopia \u201d (2014)."},{"key":"e_1_2_11_15_1","volume-title":"WHO Operational Handbook on Tuberculosis: Module 3: Diagnosis: Rapid Diagnostics for Tuberculosis Detection: Web Annex A: Information Sheets","author":"World Health Organization","year":"2024"},{"key":"e_1_2_11_16_1","unstructured":"World Health Organization \u201cAutomated Real\u2010Time Nucleic Acid Amplification Technology for Rapid and Simultaneous Detection of Tuberculosis and Rifampicin Resistance: Xpert MTB\/RIF 33 System for the Diagnosis of Pulmonary and Extra\u2010Pulmonary TB in Adults and Children: Policy Update \u201d (2014)."},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S300585"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S248059"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-024-09135-0"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s40121-020-00368-5"},{"key":"e_1_2_11_21_1","article-title":"Detection of Mycobacterium tuberculosis and Rifampicin Resistance Using GeneXpert MTB\/RIF Assay at Enat Hospital, Central Ethiopia","volume":"7","author":"Selfegna S.","year":"2022","journal-title":"Tuberculosis Research and Treatment"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2023.1266780"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-023-08469-5"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0277536"},{"issue":"2","key":"e_1_2_11_25_1","first-page":"60","article-title":"Xpert MTB\/RIF Assay for the Diagnosis of Mycobacterium tuberculosis and Its Rifampicin Resistance at Felege Hiwot and Debre Tabor Hospitals, Northwest Ethiopia: A Preliminary Implementation Research","volume":"30","author":"Derbie A.","year":"2016","journal-title":"Ethiopian Journal of Health Development"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40794-020-00115-1"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40001-022-00650-x"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1177\/20503121211045541"},{"key":"e_1_2_11_29_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874285801812010390"},{"key":"e_1_2_11_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/tmi.13489"},{"issue":"1","key":"e_1_2_11_31_1","article-title":"The Use of the Gene Xpert Mycobacterium tuberculosis\/Rifampicin (MTB\/Rif) Assay in Detection of Multi\u2010Drug Resistant Tuberculosis (MDRTB) in Nnamdi Azikiwe University Teaching Hospital, Nnewi, Nigeria","volume":"3","author":"Okonkwo R. C.","year":"2017","journal-title":"Journal of HIV & Retro Virus"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1177\/20503121221148603"},{"key":"e_1_2_11_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-021-02210-5"},{"key":"e_1_2_11_34_1","doi-asserted-by":"publisher","DOI":"10.5799\/jmid.951506"},{"issue":"5","key":"e_1_2_11_35_1","first-page":"DC46","article-title":"Rifampicin Resistance by Xpert MTB\/RIF Assay in Pulmonary Tuberculosis\u2014Is There a Need for Confirmation by Retesting?","volume":"16","author":"Kanade S. R.","year":"2022","journal-title":"Journal of Clinical and Diagnostic Research"},{"key":"e_1_2_11_36_1","first-page":"91","article-title":"Assess the Diagnostic Accuracy of GeneXpert to Detect Mycobacterium tuberculosis and Rifampicin\u2010Resistant Tuberculosis Among Presumptive Tuberculosis and Presumptive Drug Resistant Tuberculosis Patients","volume":"15","author":"Ramachandra V.","year":"2024","journal-title":"Microbiological Research"},{"key":"e_1_2_11_37_1","doi-asserted-by":"publisher","DOI":"10.4103\/ijmy.ijmy_219_22"},{"key":"e_1_2_11_38_1","article-title":"Molecular Epidemiology of Rifampicin Resistance in Mycobacterium tuberculosis Using the GeneXpert MTB\/RIF Assay From a Rural Setting in India","volume":"5","author":"Reddy R.","year":"2017","journal-title":"Journal of Pathogens"},{"key":"e_1_2_11_39_1","doi-asserted-by":"publisher","DOI":"10.4236\/jtr.2016.41007"},{"key":"e_1_2_11_40_1","doi-asserted-by":"publisher","DOI":"10.3855\/jidc.14990"},{"key":"e_1_2_11_41_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0271130"},{"key":"e_1_2_11_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.nmni.2017.07.002"},{"key":"e_1_2_11_43_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-019-4241-7"},{"key":"e_1_2_11_44_1","doi-asserted-by":"publisher","DOI":"10.2147\/IDR.S433789"},{"key":"e_1_2_11_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.heliyon.2023.e19554"},{"key":"e_1_2_11_46_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0277145"},{"key":"e_1_2_11_47_1","doi-asserted-by":"publisher","DOI":"10.21037\/atm.2016.05.09"},{"key":"e_1_2_11_48_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-024-09238-8"},{"key":"e_1_2_11_49_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12879-022-07598-7"},{"issue":"8","key":"e_1_2_11_50_1","first-page":"896","article-title":"Anti\u2010Tuberculosis Drug Resistance in Patients With HIV and Tuberculosis in South India","volume":"9","author":"Swaminathan S.","year":"2005","journal-title":"International Journal of Tuberculosis and Lung Disease"},{"key":"e_1_2_11_51_1","unstructured":"World Health Organization Guidelines Approved by the Guidelines Review Committee \u201cCompanion Handbook to the WHO Guidelines for the Programmatic Management of Drug\u2010Resistant Tuberculosis \u201d (Geneva Switzerland 2014)."}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.25111","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,11,22]],"date-time":"2024-11-22T23:56:10Z","timestamp":1732319770000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.25111"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,10]]},"references-count":50,"journal-issue":{"issue":"22","published-print":{"date-parts":[[2024,11]]}},"alternative-id":["10.1002\/jcla.25111"],"URL":"https:\/\/doi.org\/10.1002\/jcla.25111","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,10]]},"assertion":[{"value":"2024-07-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-09-22","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2024-10-10","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e25111"}}