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In the Reasons for Geographic And Racial Differences in Stroke, a longitudinal study of 30,239 Black and White U.S. adults, we investigated reliability of HbA1c measurements for two types of samples stored at \u221280\u00b0C for up to 14\u2009years.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Among 917 participants without diabetes, HbA1c was measured in 2017 in frozen packed cells from the first visit (2003\u20132007) and in frozen whole blood samples from the second visit (2013\u20132016). To study reliability, associations between HbA1c and glycemia\u2010related characteristics were examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Each 10\u2009mg\/dL greater fasting glucose was associated with 0.08% (95% CI: 0.05%\u20130.11%) greater HbA1c in frozen packed cells (Visit 1) and 0.10% (95% CI: 0.08%\u20130.12%) greater HbA1c in whole blood (Visit 2). HbA1c was also similarly higher with both methods with increasing age, gender, systolic blood pressure, body mass index, high\u2010density lipoprotein, triglycerides, C\u2010reactive protein, and hemoglobin. Using both methods, \u2264\u20093.5% would be classified with diabetes based on HbA1c\u2009\u2265\u20096.5%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In REGARDS participants without diabetes, HbA1c measurement appeared reliable in frozen packed cells or whole blood under long\u2010term storage, suggesting acceptability for study of the epidemiology of HbA1c.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.25121","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T07:55:38Z","timestamp":1739260538000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Measuring Hemoglobin <scp>A1C<\/scp> with Frozen Packed Cell and Frozen Whole Blood Samples in an Epidemiologic Study: The Reasons for Geographic and Racial Differences in Stroke Study"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5573-2573","authenticated-orcid":false,"given":"Debora","family":"Kamin Mukaz","sequence":"first","affiliation":[{"name":"Department of Medicine, Larner College of Medicine University of Vermont  Burlington Vermont USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephanie","family":"Tison","sequence":"additional","affiliation":[{"name":"Office of Data Science and Sharing Eunice Kennedy Shriver National Institute of Child Health and Human Development  Bethesda Maryland USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. Leann","family":"Long","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Data Science, School of Medicine Wake Forest University  Winston\u2010Salem North Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"April P.","family":"Carson","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Mississippi Medical Center  Jackson Mississippi USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kelly J.","family":"Hunt","sequence":"additional","affiliation":[{"name":"Department of Public Health Sciences Medical University of South Carolina  Charleston South Carolina USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne E.","family":"Judd","sequence":"additional","affiliation":[{"name":"Department of Biostatistics, School of Public Health University of Alabama at Birmingham  Birmingham Alabama 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