{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T17:10:11Z","timestamp":1700413811205},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2001,3,30]],"date-time":"2001-03-30T00:00:00Z","timestamp":985910400000},"content-version":"vor","delay-in-days":29,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2001,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Gross cystic breast disease &amp;lpar;GCBD&amp;rpar; is the most common benign disease of the human female breast, and patients with GCBD have an increased risk of breast cancer. The aim of this study was to evaluate the distribution inside apocrine cells and in breast cyst fluids aspirated from gross cysts of prostate&amp;hyphen;specific antigen &amp;lpar;PSA&amp;rpar; molecular forms, and to correlate the different intracystic PSA profiles to the subpopulations of gross cysts. Type I cysts showed a median value of 0.71 &amp;mgr;g&amp;sol;L of total PSA and 0.32 g&amp;sol;L of ACT, significantly different to that of Type II cysts &amp;lpar;Wilcoxon <jats:italic>P<\/jats:italic> &amp;lt; 0.001&amp;rpar;. Although large excesses of ACT were detected in all samples, BCF samples and apocrine cells from Type I gross cysts contained about 70&amp;percnt; of free PSA, compared to the higher amounts of complexed PSA found in Type II gross cysts. We demonstrate that in apocrine&amp;sol;secretive Type I breast gross cysts the serine protease PSA was mainly present in its free form, in contrast to a major proportion of complexed PSA found in flattened&amp;sol;transudative Type II cysts. Our results are consistent with the notion that a prolonged exposure of apocrine breast cells lining the Type I gross cysts to the proteolytic activity of PSA could be involved in the etiopathogenesis of GCBD. J. Clin. Lab. Anal. 15:81&amp;ndash;86, 2001. &amp;copy; 2001 Wiley&amp;hyphen;Liss, Inc.<\/jats:p>","DOI":"10.1002\/jcla.6","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:28:49Z","timestamp":1030310929000},"page":"81-86","source":"Crossref","is-referenced-by-count":4,"title":["Differential distribution of soluble and complexed forms of prostate&amp;hyphen;specific antigen in cyst fluids of women with gross cystic breast disease"],"prefix":"10.1002","volume":"15","author":[{"given":"Manuela","family":"Malatesta","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ferdinando","family":"Mannello","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maurizio","family":"Sebastiani","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giancarlo","family":"Gazzanelli","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2001,3,30]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Disease of the breast","author":"Haagensen CD","year":"1986"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM198210143071611"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1991.433"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.epirev.a036103"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)77655-8"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)90150-2"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1990.tb17786.x"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0960-0760(94)90276-3"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00665977"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1005932915429"},{"key":"e_1_2_1_12_2","first-page":"1791","article-title":"Association of cyst type with risk factors for breast cancer and relapse rate in women with gross cystic disease of the breast","volume":"52","author":"Naldoni C","year":"1992","journal-title":"Cancer Res"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.314.7085.925"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.315.7107.545"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)06408-3"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01806212"},{"key":"e_1_2_1_17_2","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1093\/clinchem\/41.1.7","article-title":"Nonprostatic sources of prostatic&hyphen;specific antigen: a steroid hormone&hyphen;dependent phenomenon?","volume":"41","author":"Graves HCB","year":"1995","journal-title":"Clin Chem"},{"key":"e_1_2_1_18_2","first-page":"2104","article-title":"Prostate&hyphen;specific antigen is a new favourable prognostic indicator for women with breast cancer","volume":"55","author":"Yu H","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006210627219"},{"key":"e_1_2_1_20_2","first-page":"2563","article-title":"Immunohistochemical analysis of prostate&hyphen;specific antigen does not correlate to other prognostic factors in breast cancer","volume":"19","author":"Heyl W","year":"1999","journal-title":"Anticancer Res"},{"key":"e_1_2_1_21_2","first-page":"41","article-title":"The normal female and the male breast epithelium does not express prostate&hyphen;specific antigen. Preliminary immunohistochemical observations of autopsy breast tissues","volume":"18","author":"Zaviacic M","year":"1999","journal-title":"Gen Physiol Biophys"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01806142"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01806144"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19960611)66:6<743::AID-IJC6>3.0.CO;2-#"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2825(1999)13:2<75::AID-JCLA6>3.0.CO;2-F"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1006182518520"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0277-5379(85)90289-5"},{"key":"e_1_2_1_28_2","doi-asserted-by":"crossref","first-page":"1448","DOI":"10.1093\/clinchem\/43.8.1448","article-title":"Prostate&hyphen;specific antigen expression in a case of intracystic carcinoma of the breast: characterization of immunoreactive protein and literature surveys","volume":"43","author":"Mannello F","year":"1997","journal-title":"Clin Chem"},{"key":"e_1_2_1_29_2","doi-asserted-by":"crossref","first-page":"78","DOI":"10.1093\/clinchem\/45.1.78","article-title":"Immunoreactivity, ultrastructural localization and transcript expression of prostate&hyphen;specific antigen in human neuroblastoma cell lines","volume":"45","author":"Mannello F","year":"1999","journal-title":"Clin Chem"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(94)90392-1"},{"key":"e_1_2_1_31_2","first-page":"107","article-title":"Improved electrophoretic map of breast cyst fluid proteins","volume":"16","author":"Mannello F","year":"1992","journal-title":"Cancer Detect Prev"},{"key":"e_1_2_1_32_2","first-page":"6379","article-title":"Proteases in cyst fluid from human gross cyst breast disease","volume":"48","author":"Kesner L","year":"1988","journal-title":"Cancer Res"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/0959-8049(92)90124-K"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.80.5.1515"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.75.4.1046"},{"key":"e_1_2_1_36_2","doi-asserted-by":"crossref","first-page":"940","DOI":"10.1006\/bbrc.1993.1506","article-title":"Mitogenic response of osteoblast cells to prostate&hyphen;specific antigen suggests an activation of latent TGF&hyphen;&bgr; and proteolytic modulation of cell adhesion receptors","volume":"92","author":"Killian CS","year":"1993","journal-title":"Biochem Biophys Res Commun"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910630618"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6692167"},{"key":"e_1_2_1_39_2","first-page":"2805","article-title":"Transforming growth factor alpha, beta 1 and beta 2 in breast cyst fluid","volume":"14","author":"Lai LC","year":"1994","journal-title":"Anticancer Res"},{"key":"e_1_2_1_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(01)66399-7"},{"key":"e_1_2_1_41_2","doi-asserted-by":"crossref","first-page":"755","DOI":"10.1093\/clinchem\/45.6.755","article-title":"Prostate&hyphen;specific antigen: advances and challenges","volume":"45","author":"Chan DW","year":"2000","journal-title":"Clin Chem"},{"key":"e_1_2_1_42_2","doi-asserted-by":"crossref","first-page":"896","DOI":"10.1093\/clinchem\/46.7.896","article-title":"Prostate&hyphen;specific antigen: a cancer fighter and a valuable messenger?","volume":"46","author":"Diamandis EP","year":"2000","journal-title":"Clin Chem"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fjcla.6","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.6","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,19]],"date-time":"2023-11-19T16:49:05Z","timestamp":1700412545000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2001,3]]},"references-count":41,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2001,3]]}},"alternative-id":["10.1002\/jcla.6"],"URL":"https:\/\/doi.org\/10.1002\/jcla.6","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2001,3]]}}}