{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T18:28:09Z","timestamp":1789583289188,"version":"build-2803163510"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,2,10]],"date-time":"2025-02-10T00:00:00Z","timestamp":1739145600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The Philadelphia chromosome (Ph) represented a finding of chronic myeloid leukemia (CML) in most cases which formed from t (9; 22) (q34; q11) resulting in the Breakpoint cluster region\u2010Abelson tyrosine\u2010protein kinase1 (BCR\u2010ABL1) fusion gene. Assuming CCE's inaccuracies in diagnosing CML and FISH's limitations with low BCR\u2010ABL1 percentages, a Predicted\u2010FISH (Pred\u2010FISH) was developed. This model predicts treatment response during follow\u2010up by integrating qRT\u2010PCR results, White Blood Cell (WBC) counts, and Cytogenetic Response data.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Quantitative Real\u2010Time Polymerase Chain Reaction Analysis (qRT\u2010PCR), fluorescence in\u00a0situ hybridization (FISH), and Conventional Cytogenetic Examination (CCE or Karyotyping) have been used in the detection and follow\u2010up of CML patients. The study included 110 individuals, divided into three groups: 31.82% (35 individuals) were newly diagnosed CML patients, another 22.73% (25 individuals) were healthy control samples, and the remaining 45.45% (50 individuals) were previously diagnosed CML patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Include BCR\u2010ABL1 fusion gene levels detected by qRT\u2010PCR, Ph chromosome presence t (9; 22) (q34; q11) observed by CCE, and WBC counts. The FISH test, used to confirm disease in new patients before treatment, was compared to CCE results due to its insensitivity in certain conditions. Data from CCE, FISH, qRT\u2010PCR, and WBC for newly diagnosed patients provided a standard for evaluating the Predicted\u2010FISH.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The FISH technique excels in disease detection with over 98% accuracy and high sensitivity. QRT\u2010PCR is effective for monitoring CML and BCR\u2010ABL1 gene levels, indicating MMR and DMR. CCE, while useful for posttreatment monitoring, is less accurate in measuring treatment response over time.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.70001","type":"journal-article","created":{"date-parts":[[2025,2,11]],"date-time":"2025-02-11T04:29:40Z","timestamp":1739248180000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Improving the Diagnosis and Follow\u2010Up of Chronic Myeloid Leukemia Using Conventional and Molecular Techniques"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-3323-4067","authenticated-orcid":false,"given":"Noor Al\u2010Huda A.","family":"Bahar","sequence":"first","affiliation":[{"name":"College of Medicine, Department of Microbiology University of Anbar  Ramadi Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0454-8351","authenticated-orcid":false,"given":"Mushtak T. S.","family":"Al\u2010Ouqaili","sequence":"additional","affiliation":[{"name":"College of Medicine, Department of Microbiology University of Anbar  Ramadi Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0002-3831-1720","authenticated-orcid":false,"given":"Nabeel M.","family":"Talib","sequence":"additional","affiliation":[{"name":"Anbar Health Directorate Anbar Cancer Center  Ramadi Iraq"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,2,10]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.7326\/aimcc.2022.0116"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.15761\/CCRR.1000348"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmoldx.2019.03.002"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/cancers15010300"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.5935\/1676\u20102444.20180015"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.12801"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.leukres.2013.10.005"},{"key":"e_1_2_10_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102006\u201001\u20100092"},{"key":"e_1_2_10_10_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102013\u201005\u2010501569"},{"key":"e_1_2_10_11_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12920\u2010023\u201001607\u20107"},{"key":"e_1_2_10_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102011\u201010\u2010388702"},{"key":"e_1_2_10_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/isbn.978-3-318-06867-2"},{"key":"e_1_2_10_14_1","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009207601835"},{"key":"e_1_2_10_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-52959-1_24"},{"key":"e_1_2_10_16_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2021.720507"},{"key":"e_1_2_10_17_1","doi-asserted-by":"publisher","DOI":"10.5339\/jemtac.2022.aimco.8"},{"key":"e_1_2_10_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/rda.14100"},{"key":"e_1_2_10_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00277\u2010015\u20102322\u20102"},{"key":"e_1_2_10_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365\u20102141.2011.08603.x"},{"key":"e_1_2_10_21_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood\u20102016\u201001\u2010694440"},{"key":"e_1_2_10_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010018\u20100323\u20102"},{"key":"e_1_2_10_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41375\u2010020\u20100776\u20102"},{"key":"e_1_2_10_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/JOURNAL.PONE.0222360"},{"key":"e_1_2_10_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/plants12010080"},{"key":"e_1_2_10_26_1","unstructured":"A.YasmeenandA.Saleh \u201cMolecular Study of BCR\u2010ABL Gene and Genetic Variations in Chronic Myeloid Leukemia Patients in Iraq \u201d (Thesis College of Science\/AL\u2010Nahrain University 2011 42\u201043) https:\/\/nahrainuniv.edu.iq\/sites\/default\/files\/yasmin.pdf."},{"key":"e_1_2_10_27_1","doi-asserted-by":"publisher","DOI":"10.32894\/kjms.2021.169390"},{"key":"e_1_2_10_28_1","doi-asserted-by":"publisher","DOI":"10.1080\/10428190902838384"},{"key":"e_1_2_10_29_1","doi-asserted-by":"publisher","DOI":"10.3760\/CMA.J.ISSN.0253\u20102727.2017.11.012"},{"key":"e_1_2_10_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/09553002.2024.2356545"},{"key":"e_1_2_10_31_1","doi-asserted-by":"publisher","DOI":"10.3892\/br.2017.967"},{"issue":"2","key":"e_1_2_10_32_1","first-page":"149","article-title":"FISH Versus Real\u2010Time Quantitative PCR for Monitoring of Minimal Residual Disease in Chronic Myeloid Leukaemia Patients on Tyrosine Kinase Inhibitor Therapy","volume":"41","author":"Haidary A. 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