{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,11]],"date-time":"2026-06-11T10:23:22Z","timestamp":1781173402555,"version":"3.54.1"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2025,2,28]],"date-time":"2025-02-28T00:00:00Z","timestamp":1740700800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>This study aimed to evaluate the diagnostic ability of interleukin 2 (IL\u20102) and interferon gamma (IFN\u2010\u03b3) release assay induced by the fusion protein (ESAT\u20106\/CFP\u201010\/Rv1985c) for detecting active tuberculosis (ATB) in clinically visiting patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 970 subjects (215 in ATB group and 755 in non\u2010ATB group) underwent both an interferon\u2010\u03b3 release assay (IGRA) and a TB\u2010DNA PCR assay. Using clinical diagnosis as the gold standard, both qualitative and quantitative test results for IL\u20102 and IFN\u2010\u03b3 were analyzed. Subsequently, the diagnostic ability of IL\u20102 and IFN\u2010\u03b3 to screen for ATB among the high\u2010risk population was then evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>IL\u20102 exhibited higher specificity, while IFN\u2010\u03b3 demonstrated higher sensitivity in distinguishing between ATB and non\u2010ATB subjects. The sensitivity of the serial application of IL\u20102 and IFN\u2010\u03b3 had no significant difference (<jats:italic>p<\/jats:italic>\u2009=\u20091.000) compared with IFN\u2010\u03b3; the specificity of the serial application of IL\u20102 and IFN\u2010\u03b3 had no significant difference (<jats:italic>p<\/jats:italic>\u2009=\u20090.708) compared with IL\u20102. Quantitative analysis of the results revealed that the IL\u20102 and IFN\u2010\u03b3 values were significantly higher in the ATB group compared with the non\u2010ATB group. Additionally, the combined predictors of IL\u20102 and IFN\u2010\u03b3 did not show a significant difference compared with IL\u20102 alone (<jats:italic>p<\/jats:italic>\u2009=\u20090.324) or IFN\u2010\u03b3 alone (<jats:italic>p<\/jats:italic>\u2009=\u20090.405).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This study demonstrated that IL\u20102 and IFN\u2010\u03b3 release assays induced by the fusion protein (ESAT\u20106\/CFP\u201010\/Rv1985c) were valuable for distinguishing ATB from non\u2010ATB subjects, with IL\u20102 exhibiting higher specificity and IFN\u2010\u03b3 demonstrating higher sensitivity.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.70010","type":"journal-article","created":{"date-parts":[[2025,3,11]],"date-time":"2025-03-11T15:06:15Z","timestamp":1741705575000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["The Diagnostic Value of Interleukin\u20102 and Interferon\u2010\u03b3 Induced by Fusion Protein (<scp>ESAT<\/scp>\u20106\/<scp>CFP<\/scp>\u201010\/Rv1985c) for Active <i>Mycobacterium tuberculosis<\/i> Infection"],"prefix":"10.1002","volume":"39","author":[{"given":"Zhipeng","family":"Zhao","sequence":"first","affiliation":[{"name":"Laboratory Medicine Department of Beijing Tsinghua Changgung Hospital, School of Clinical Medicine Tsinghua University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3139-2871","authenticated-orcid":false,"given":"Runqing","family":"Li","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Department of Beijing Tsinghua Changgung Hospital, School of Clinical Medicine Tsinghua University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5486-2480","authenticated-orcid":false,"given":"Xiuying","family":"Zhao","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Department of Beijing Tsinghua Changgung Hospital, School of Clinical Medicine Tsinghua University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yujie","family":"Wang","sequence":"additional","affiliation":[{"name":"Medical Data Science Center of Beijing Tsinghua Changgung Hospital, School of Clinical Medicine Tsinghua University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minggui","family":"Lin","sequence":"additional","affiliation":[{"name":"Infectious Disease Department of Beijing Tsinghua Changgung Hospital, School of Clinical Medicine Tsinghua University  Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Wei","sequence":"additional","affiliation":[{"name":"Laboratory Medicine Department of Beijing Tsinghua Changgung Hospital, School of Clinical Medicine Tsinghua University  Beijing 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