{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T11:47:31Z","timestamp":1792324051905,"version":"4.4.0"},"reference-count":22,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T00:00:00Z","timestamp":1744848000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Thyroid disorders affect millions of people around the world, mainly women. Measurement of hormones is critical in their identification, treatment, and monitoring; Therefore, the methods must be as reliable and valid as possible.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We designed a cross\u2010sectional study to compare thyroid\u2010stimulating hormone (TSH) and free tetraiodothyronine (fT4) concentrations using ELISA (Enzyme\u2010Linked Immunosorbent Assay) and LFA (Lateral Flow Assay) in human serum samples. We obtained 96 serum samples that were evaluated with a commercial kit for ELISA (Accubind Monobite, Lake Forest, California, US) and LFA (Micropofit Fluorecare, Guangdong, Shenzhen, China). We used the Wilcoxon nonparametric test, Bland\u2013Altman, and Passing\u2010Bablok regression to compare TSH and fT4 concentrations obtained by ELISA and LFA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The median TSH concentrations obtained by ELISA and LFA were 1.92 and 2.11 \u03bcIU\/mL, and for fT4 were 1.14 and 1.10\u2009ng\/dL. On the other hand, the Spearman's rho between ELISA and LFA for TSH was 0.845. The TSH concentrations between ELISA and LFA had significant differences (<jats:italic>p<\/jats:italic>\u2009&lt;\u20090.05). About fT4, the Spearman's rho between methods was 0.348. The bias for TSH was \u22120.315, and for fT4 was \u22120.013. The Deming regression for TSH (<jats:italic>p<\/jats:italic>\u2009=\u20090.309) and fT4 (=0.938) shows that the levels obtained by both methods do not present significant differences, and the Passing\u2010Bablok regression identifies significant bias between both methods, especially for fT4, in the range of concentrations studied.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TSH measurement by LFA may be a viable alternative to evaluate thyroid diseases, but fT4 by LFA is not precise and presents a high bias.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.70032","type":"journal-article","created":{"date-parts":[[2025,4,17]],"date-time":"2025-04-17T16:05:06Z","timestamp":1744905906000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparison of Enzyme\u2010Linked Immunosorbent Assay and Lateral Flow Assay to Measure Thyroid\u2010Stimulating Hormone and Free <scp>T4<\/scp> in Human Serum"],"prefix":"10.1002","volume":"39","author":[{"given":"Rolando","family":"Adv\u00edncula\u2010Espino","sequence":"first","affiliation":[{"name":"Facultad de Farmacia y Bioqu\u00edmica, Universidad Nacional Mayor de san Marcos  Lima Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jair","family":"Li","sequence":"additional","affiliation":[{"name":"Facultad de Tecnolog\u00eda M\u00e9dica, Universidad Nacional Federico Villarreal  Lima Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaime","family":"Rosales\u2010Rimache","sequence":"additional","affiliation":[{"name":"Carrera de Medicina, Universidad Continental  Huancayo Peru"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,4,17]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.otc.2018.01.014"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.3121\/cmr.2016.1309"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/438037"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.1530\/EJE\u201016\u20100193"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1159\/000184776"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.22667"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1563-2571.2000.270207.x"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.1159\/000507018"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1089\/thy.1992.2.237"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.1258\/0969141042467340"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2009.140178"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2009.140194"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2009.140228"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.291.2.239"},{"issue":"3","key":"e_1_2_12_16_1","first-page":"155","article-title":"Existing and Emerging Technologies for Point\u2010Of\u2010Care Testing","volume":"35","author":"St John A.","year":"2014","journal-title":"Clinical Biochemist Reviews"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.7754\/Clin.Lab.2016.160601"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.talanta.2020.120961"},{"key":"e_1_2_12_19_1","doi-asserted-by":"publisher","DOI":"10.2174\/1874944501912010342"},{"issue":"4","key":"e_1_2_12_20_1","first-page":"258","article-title":"Clinical Usefulness of ELISA Technique in the Assessment of Thyroid Function","volume":"9","author":"Agharanya J. C.","year":"2000","journal-title":"West African Journal of Medicine"},{"key":"e_1_2_12_21_1","doi-asserted-by":"publisher","DOI":"10.3390\/diagnostics11091590"},{"key":"e_1_2_12_22_1","doi-asserted-by":"publisher","DOI":"10.1080\/15321819.2021.1891931"},{"key":"e_1_2_12_23_1","doi-asserted-by":"publisher","DOI":"10.11613\/bm.2011.010"}],"container-title":["Journal of Clinical Laboratory Analysis"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/jcla.70032","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,5,20]],"date-time":"2025-05-20T10:51:57Z","timestamp":1747738317000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/jcla.70032"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,4,17]]},"references-count":22,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2025,5]]}},"alternative-id":["10.1002\/jcla.70032"],"URL":"https:\/\/doi.org\/10.1002\/jcla.70032","archive":["Portico"],"relation":{},"ISSN":["0887-8013","1098-2825"],"issn-type":[{"value":"0887-8013","type":"print"},{"value":"1098-2825","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,4,17]]},"assertion":[{"value":"2024-12-02","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-03-29","order":2,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2025-04-17","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e70032"}}