{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T20:04:23Z","timestamp":1786565063342,"version":"build-2736575974"},"reference-count":24,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T00:00:00Z","timestamp":1744934400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The increasing prevalence of infections caused by carbapenem\u2010resistant organisms (CRO) represents a global health issue. Therefore, a rapid and accurate method for detecting these microbes in any clinical microbiology laboratory is crucial for the prevention and control of their transmission, as well as for clinical treatment. This study aimed to evaluate the phenotypic detection methods, PBA\u2010EDTA, mCIM, PCR, and immunocolloidal gold kit for CRO.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We collected 99 samples from the inpatients in Sun Yat\u2010Sen University Cancer Center (SYSUCC) from March 2019 to February 2022 and classified the drug resistance and genotype of various strains by various enzyme\u2010type experiments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Out of 99 multidrug\u2010resistant Gram\u2010negative bacilli resistant to carbapenems, 58 (58.59%) were identified as carbapenemase\u2010positive using the mCIM test. The carbapenemase genotypes included 19 NDM strains, 4 KPC strains, 1 IMP strain, and 5 OXA\u201023 strains. Enzyme detection revealed 21 strains positive for metallo\u2010\u03b2\u2010lactamase, 50 for serine\u2010\u03b2\u2010lactamase, and 2 positive for both, with a total positive rate of 73.74%. 26 strains were negative for enzyme detection, and mCIM showed limited effectiveness in detecting strains coproducing NDM and KPC. The immunocolloidal gold assay had a sensitivity of 96.9% and specificity of 98.5%.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study used an immunocolloidal gold kit for carbapenemase detection, providing results within 15\u2009min. This cost\u2010effective method can quickly assist in identifying carbapenemase genotypes and holds potential as a new rapid detection and diagnostic tool for CRE in clinical and lab settings.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.70038","type":"journal-article","created":{"date-parts":[[2025,4,18]],"date-time":"2025-04-18T12:20:02Z","timestamp":1744978802000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Phenotype Detection and Drug Resistance Analysis of Carbapenem\u2010Resistant Gram\u2010Negative Bacilli"],"prefix":"10.1002","volume":"39","author":[{"given":"Xiao\u2010min","family":"Liu","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory State Key Laboratory of Oncology in South China, Guangdong Key Laboratory of Nasopharyngeal Carcinoma Diagnosis and Therapy, Guangdong Provincial Clinical Research Center for Cancer, Sun Yat\u2010Sen University Cancer Center  Guangzhou P. R. 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