{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T01:37:03Z","timestamp":1780364223943,"version":"3.54.1"},"reference-count":45,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T00:00:00Z","timestamp":1747353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Clinical Laboratory Analysis"],"published-print":{"date-parts":[[2025,6]]},"abstract":"ABSTRACT<\/jats:title>Background<\/jats:title>Colorectal cancer (CRC<\/jats:styled-content>) is a worldwide burden. Circular RNAs<\/jats:styled-content> are promising biomarkers for diagnosing and prognosis of CRC<\/jats:styled-content>.<\/jats:p><\/jats:sec>Objective<\/jats:title>To investigate the possible association of sera levels of CircFUNDC1<\/jats:styled-content> and CircUHRF1<\/jats:styled-content> expression with predisposition and clinicopathological findings in CRC<\/jats:styled-content>, ulcerative colitis (UC<\/jats:styled-content>), and Crohn's disease (CD<\/jats:styled-content>) in Egyptian patients.<\/jats:p><\/jats:sec>Methods<\/jats:title>The serum levels of CircFUNDC1<\/jats:styled-content> and CircUHRF1<\/jats:styled-content> were evaluated in 113 Egyptian subjects divided into four groups; CRC<\/jats:styled-content> (31), UC<\/jats:styled-content> (26), and CD<\/jats:styled-content> (25) and compared to healthy controls (31) using quantitative polymerase chain reaction.<\/jats:p><\/jats:sec>Results<\/jats:title>The median values of log2 serum fold change (FC<\/jats:styled-content>) of CircFUNDC1<\/jats:styled-content> in CRC<\/jats:styled-content>, UC<\/jats:styled-content>, and CD<\/jats:styled-content> patients were 9.11, 6.58, and 6.17, respectively. It was upregulated in all case groups. CRC<\/jats:styled-content>, UC<\/jats:styled-content>, and CD<\/jats:styled-content> patients had significantly higher serum CircFUNDC1<\/jats:styled-content> levels than controls (p<\/jats:italic>\u2009<\u20090.001). However, there were no significant differences among patient groups (CRC<\/jats:styled-content>, UC<\/jats:styled-content>, and CD<\/jats:styled-content>). The medians of log 2 of serum FC CircUHRF1<\/jats:styled-content> in patients with CRC<\/jats:styled-content>, UC<\/jats:styled-content>, and CD<\/jats:styled-content> were \u22122.00, 3.33, and 3.12, respectively. The CircUHRF1<\/jats:styled-content> serum level was lower in the CRC<\/jats:styled-content> group of patients, with no significant difference between the CRC<\/jats:styled-content> group and the controls. Serum CircUHRF1<\/jats:styled-content> was significantly overexpressed in patients with UC<\/jats:styled-content> and CD<\/jats:styled-content> compared to the CRC<\/jats:styled-content> groups or controls (p<\/jats:italic>\u2009<\u20090.001). By Roc curve analysis, both genes can differentiate CRC<\/jats:styled-content> patients from inflammatory bowel disease (IBD<\/jats:styled-content>) patients or healthy controls with p<\/jats:italic>\u2009<\u20090.05.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Serum CircFUNDC1<\/jats:styled-content> is a biomarker for CRC<\/jats:styled-content>, while CircUHRF1<\/jats:styled-content> is a biomarker of IBD<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1002\/jcla.70039","type":"journal-article","created":{"date-parts":[[2025,5,16]],"date-time":"2025-05-16T09:55:27Z","timestamp":1747389327000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Expression Profile of Serum CircFUNDC1<\/scp> and CircUHRF1<\/scp> Can Differentiate Between Colorectal Cancer and Inflammatory Bowel Diseases (Ulcerative Colitis and Crohn's Disease)"],"prefix":"10.1002","volume":"39","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4007-7868","authenticated-orcid":false,"given":"Marwa A.","family":"Ali","sequence":"first","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine Fayoum University Fayoum Egypt"},{"name":"Department of Biomedical Sciences, College of Medicine King Faisal University Al\u2010Ahsa Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3031-3599","authenticated-orcid":false,"given":"Olfat G.","family":"Shaker","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine Cairo University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eman M.","family":"Ezzat","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, Faculty of Medicine Fayoum University Fayoum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abeer A.","family":"Khalefa","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Zagazig University Zagazig Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naglaa A.","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Physiology, Faculty of Medicine Zagazig University Zagazig Egypt"},{"name":"Department of Physiology, College of Medicine Najran University Najran Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raghda Yahia","family":"Abu El\u2010Ela","sequence":"additional","affiliation":[{"name":"Department of Clinical Oncology, Faculty of Medicine Fayoum University Fayoum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amal A. Ibrahim","family":"Amin","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Faculty of Medicine Fayoum University Fayoum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatma Abdel Hamid","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Department of Tropical Medicine Faculty of Medicine, Fayoum University Fayoum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa Mamdouh Ahmed","family":"Abdelhafeez","sequence":"additional","affiliation":[{"name":"Department of Clinical and Chemical Pathology, Faculty of Medicine Fayoum University Fayoum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed Saad Zaghloul","family":"Ahmed","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Immunology, Faculty of Medicine Fayoum University Fayoum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marwa N.","family":"AbdelHafez","sequence":"additional","affiliation":[{"name":"Department of Medical Oncology, National Cancer Institute Cairo University Cairo Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdulrhman Khaled Al","family":"Abdulqader","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine, College of Medicine King Faisal University Al\u2010Ahsa Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reham","family":"Fares","sequence":"additional","affiliation":[{"name":"Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine Fayoum University Fayoum Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,5,16]]},"reference":[{"key":"e_1_2_12_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21660"},{"key":"e_1_2_12_3_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2022.07.066"},{"key":"e_1_2_12_4_1","doi-asserted-by":"publisher","DOI":"10.14309\/ajg.0000000000000152"},{"key":"e_1_2_12_5_1","doi-asserted-by":"publisher","DOI":"10.21037\/tgh\u201023\u201036"},{"key":"e_1_2_12_6_1","doi-asserted-by":"publisher","DOI":"10.1093\/ecco\u2010jcc\/jjae061"},{"key":"e_1_2_12_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.21365"},{"key":"e_1_2_12_8_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2020.12.036"},{"key":"e_1_2_12_9_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25115699"},{"key":"e_1_2_12_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2023.115217"},{"key":"e_1_2_12_11_1","doi-asserted-by":"publisher","DOI":"10.3389\/fneur.2022.846198"},{"key":"e_1_2_12_12_1","doi-asserted-by":"publisher","DOI":"10.1126\/SCISIGNAL.AAZ8240"},{"key":"e_1_2_12_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598\u2010022\u201023463\u20108"},{"key":"e_1_2_12_14_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943\u2010020\u201001222\u20105"},{"key":"e_1_2_12_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12876\u2010023\u201002968\u20100"},{"key":"e_1_2_12_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/crocol\/otab007"},{"key":"e_1_2_12_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ncrna.2024.01.012"},{"key":"e_1_2_12_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjid.2022.102354"},{"issue":"1","key":"e_1_2_12_19_1","first-page":"1127","article-title":"Clinicopathological Profile of Colorectal Cancer Patients in Suez Canal University Hospitals\u2010Egypt","volume":"8","author":"Suliman M. 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